化学学报 ›› 2011, Vol. 69 ›› Issue (23): 2859-2864.DOI: 10.6023/A1104276 上一篇    下一篇

研究论文

金纳米粒子作探针共振瑞利散射光谱法测定牛奶中三聚氰胺

曾国平1,2, 向东山2, 李丽2, 何治柯*,2   

  1. (1湖北第二师范学院 武汉 430205)
    (2武汉大学化学与分子科学学院 生物医学分析化学教育部重点实验室 武汉 430072)
  • 收稿日期:2011-04-27 修回日期:2011-06-28 出版日期:2011-12-14 发布日期:2011-07-14
  • 通讯作者: 何治柯 E-mail:zhkhe@whu.edu.cn
  • 基金资助:

    国家自然科学基金

Resonance Rayleigh Scattering Spectra Method for Detection of Melamine in Milk with Gold Nanoparticles as Probe

Zeng Guoping1,2; Xiang Dongshan2; Li Li 2; He Zhike*,2   

  1. (1 Hubei University of Education, Wuhan 430205)
    (2 Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)
  • Received:2011-04-27 Revised:2011-06-28 Online:2011-12-14 Published:2011-07-14

在PBS缓冲体系中, 金纳米粒子与三聚氰胺形成粒径较大的聚集体, 导致共振瑞利散射(RRS)强度显著增强, 在一定条件下, 散射强度(ΔIRRS)与三聚氰胺浓度成正比, 由此建立了以金纳米粒子作光谱探针检测鲜牛奶中三聚氰胺的新方法. 在优化的实验条件下, 当三聚氰胺的浓度为3.3×10-8~4.7×10-7 mol/L时, 其线性关系为: ΔI RRS=106.98C-28.279, R2=0.9971, 检出限为2.7×10-8 mol/L. 本方法金纳米粒子无需修饰, 在体系中仅加入一定量的单链寡聚核苷酸(T10), 实验表明该方法具有简便、快速、灵敏度高及选择性好等特点.

关键词: 三聚氰胺, 金纳米粒子, 共振瑞利散射

A new resonance Rayleigh scattering method was developed for the determination of melamine in milk using gold nanoparticles as spectral probe. Under certain conditions, melamine can form bigger aggregate with gold nanoparticles, which resulted in great enhancement of resonance Rayleigh scattering. Based on optimizing various parameters, the linear dynamic range, the Regression equation, the linear correlation and the detection limit were 3.3×10-8~4.7×10-7 mol/L, ΔIRRS=106.98C-28.279, R2=0.9971 and 2.7×10-8 mol/L, respectively. In this method, the gold nanoparticles do not need to be decorated. Only an amount of single-strand oligonucleotide (T10) need to be added to the system. The experiments showed that this developed method is practical, simple, sensitive and selective.

Key words: melamine, gold nanoparticles, resonance Rayleigh scattering