化学学报 ›› 2021, Vol. 79 ›› Issue (6): 694-704.DOI: 10.6023/A21010017 上一篇    下一篇

综述

表面增强拉曼光谱技术在microRNA检测中的研究进展

易荣楠a, 吴燕b,*()   

  1. a 湖南警察学院刑事科学技术系 长沙 410138
    b 长江师范学院化学化工学院 重庆 408100
  • 投稿日期:2021-01-19 发布日期:2021-03-30
  • 通讯作者: 吴燕
  • 作者简介:

    易荣楠, 男, 1992年生, 主要从事拉曼光谱技术在毒物毒品中的分析检验、有机合成方法学等方面的研究. 作为核心成员参与国家自然科学基金项目2项, 发表SCI论文8篇.

    吴燕副教授, 女, 1988年生, 主要研究方向为SERS技术及生物传感器的应用研究. 主持国家自然科学基金、重庆市科委项目、重庆市教委项目、长江师范学院青年人才成长支持计划项目、长江师范学院科研启动项目等6项. 作为骨干成员参与多项国家自然科学基金、重庆市自然科学基金等项目的研究. 近年来, 以第一作者与通讯作者发表SCI论文12篇, 授权发明专利1项, 发表教改论文2篇.

  • 基金资助:
    国家自然科学基金(21804011)

Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA

Rongnan Yia, Yan Wub()   

  1. a Criminal Technology Department, Hunan Police Academy, Changsha 410138, China
    b College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China
  • Received:2021-01-19 Published:2021-03-30
  • Contact: Yan Wu
  • Supported by:
    National Natural Science Foundation of China(21804011)

microRNA是一段长约为18~24个核苷酸的内源性非编码单链RNA. 最新研究发现: 许多疾病和肿瘤的发生与microRNA的表达水平息息相关, 且microRNA有望成为新型肿瘤标志物及癌症治疗的新目标. 因此, 发展高灵敏度、高特异性及简单快速的microRNA分析检测方法对于生物医学研究和癌症的早期诊断具有重要的意义. 表面增强拉曼光谱(SERS)技术由于具有灵敏度高、检测速度快、指纹识别、水干扰小等独特优势, 在癌症的早期诊断领域具有很大的应用价值. 作者综述了SERS技术在microRNA检测方面的最新研究进展, 分析了该技术在生物检测中亟待解决的关键问题和挑战, 并对其未来的发展前景进行了展望.

关键词: 表面增强拉曼光谱, microRNA, 肿瘤标志物

microRNA is an endogenous non-coding single-stranded RNA with a length of about 18~24 nucleotides. The latest research has found that the occurrence of many diseases and tumors is closely related to the level of microRNA expression, and microRNA is expected to become a new tumor marker and a new target for cancer treatment. Therefore, the development of high sensitivity, high specificity, and simple and rapid microRNA detection methods is greatly significant for biomedical research and early diagnosis of cancer. Surface-enhanced Raman spectroscopy (SERS) technique has great application value in the field of early cancer diagnosis due to its unique advantages such as high sensitivity, fast detection speed, fingerprint recognition, and low water interference. The latest research progress of SERS technique in microRNA detection is summarized in this review. Finally the main challenges of SERS technique in bioassay are discussed and the future development trend is proposed.

Key words: surface-enhanced Raman spectroscopy, microRNA, tumor marker