化学学报 ›› 2004, Vol. 62 ›› Issue (22): 2239-2243. 上一篇    下一篇

研究论文

DNA和菲啶鎓复合物嵌入水滑石的研究

李文卓1, 陆军2, 秦成刚1, 陈接胜1, 李连生1, 黄佰渠2   

  1. 1. 吉林大学无机合成与制备化学国家重点实验室, 长春, 130012;
    2. 东北师范大学遗传和细胞研究所, 长春, 130024
  • 收稿日期:2004-05-24 修回日期:2004-07-16 出版日期:2004-11-28 发布日期:2014-02-17
  • 通讯作者: 陈接胜,E-mail:chemcj@mail.jlu.edu.cn;Fax:(+86)0431-5671974 E-mail:chemcj@mail.jlu.edu.cn

Study on Intercalation of DNA-Ethidium Complex into Layered Hydrotalcite

LI Wen-Zhuo1, LU Jun2, QIN Cheng-Gang1, CHEN Jie-Sheng1, LI Lian-Sheng1, HUANG Bai-Qu2   

  1. 1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012;
    2. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024
  • Received:2004-05-24 Revised:2004-07-16 Online:2004-11-28 Published:2014-02-17

平面状生物荧光探针分子溴化乙锭(溴化3,8-二氨基-5-乙基-6-苯基菲啶(钅必羽),3,8-diamino-5-ethyl-6-phenyl phenanthridinium boromide, 简称Ethidium Bromide或EB)与双螺旋状的脱氧核糖核酸分子DNA相互作用生成DNA-ET (Ethidium又称菲啶,简写ET)复合物.将镁铝水滑石(HT)煅烧后的产物与DNA-ET复合物作用,得到一种新复合化合物HT-DNA-ET.经X射线衍射分析,在HT-DNA-ET中,DNA-ET复合物已嵌入到HT无机层板间.根据增大的层间距及DNA和ET的分子结构,可判断HT-DNA-ET复合化合物具有夹心型结构,两层DNA-ET复合物平行组装在HT层板间.圆二色谱和红外光谱也证实层间DNA-ET复合物的存在.复合化合物HT-DNA-ET在λex=488 nm激光激发下,发出λem,max=610 nm的荧光.与在同样条件下DNA-ET复合物发出的荧光相比,复合化合物HT-DNA-ET的λem, max向低波长方向移动了29 nm.

关键词: DNA, 溴化乙锭(EB), 水滑石, 嵌入, 发光

A new composite compound HT-DNA-ET has been prepared through intercalation of ethidium and DNA molecule into the layers of hydrotalcite (HT). The HT-DNA-ET compound has been investigated by various analytical methods such as X-ray diffraction, IR, UV-vis and circular dichroism spectroscopy, element and thermogravimetric analyses. The interlayer distance of the HT-DNA-ET compound is 4.6 nm, larger than that of the pristine HT. Taking into account the structure of the DNA molecule and the ET molecule, it was proposed that there are two layers of DNA-ET composite between two adjacent HT sheets. The DNA molecules in the HT-DNA-ET compound retain their chemical and biological integrity. The HT-DNA-ET compound gave rise to photoluminescence of λem,max=610 nm under the excitation of a laser light of λex=488 nm.

Key words: DNA, ethidium bromide(HT), layed hydroxide, intercalation, photoluminescence