化学学报 ›› 2005, Vol. 63 ›› Issue (6): 497-502. 上一篇    下一篇

研究论文

钌(II)多吡啶配合物光断裂DNA的实验研究

徐宏*,1,3,d,李浩2,刘昀2,莫重英3,梁毅3,刘剑洪1,张黔玲1,计亮年d   

  1. (1深圳大学师范学院化学与生物学系 深圳518060)
    (2深圳大学生命科学学院 深圳市微生物基因工程重点实验室 深圳 518060)
    (3武汉大学生命科学学院 病毒学教育部重点实验室 武汉 430072)
    (4南京大学配位化学国家重点实验室 南京 210093)
  • 收稿日期:2004-06-01 修回日期:2004-11-16 出版日期:2005-03-28 发布日期:2010-12-10
  • 通讯作者: 徐宏

Experimental Studies on Photocleavage of DNA by Ruthenium(II) Polypyridyl Complexes

XU Hong*,1,3,4, LI Hao2, LIU Yun2, MO Chong-Ying3, LIANG Yi3, LIU Jian-Hong1, ZHANG Qian-Ling1, JI Liang-Nian4   

  1. (1 Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060)
    (2 Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060)
    (3 Key Laboratory of Ministry of Education for Virology, College of Life Sciences, Wuhan University, Wuhan 430072)
    (4 State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093)
  • Received:2004-06-01 Revised:2004-11-16 Online:2005-03-28 Published:2010-12-10
  • Contact: XU Hong

研究了一系列钌(II)多吡啶配合物对pBR 322 DNA 的光断裂作用, 并与光谱法和粘度法的研究结果进行了对比. 实验结果表明, 钌(II)多吡啶配合物光断裂DNA的能力不仅与配合物与DNA相互作用的结合模式和结合强度有关, 还与配合物自身的电子结构有关; 钌(II)多吡啶配合物对DNA的光断裂存在立体选择性; 其断裂机理是激发态的配合物与溶液中的氧分子发生能量转移生成单线态氧活性氧化物种, 将鸟嘌呤碱基氧化而导致DNA断裂. 本研究对于遗传工程中的化学核酸酶以及以DNA为靶标的药物设计有重要的意义.

关键词: 钌(II)多吡啶配合物, DNA, 光断裂, 化学核酸酶

Photoactivated cleavage of pBR 322 DNA by a series of ruthenium(II) polypyridyl complexes has been studied. The experimental results were compared with our previous observations using spectroscopic methods and viscosity measurements. The experimental results suggest that the capacity of ruthenium(II) polypyridyl complex to photocleavage DNA depends on not only the mode and strength of the complex binding to DNA, but also the electron structure of the complex, and that ruthenium(II) polypyridyl complex can photocleavage DNA enantioselectively. The mechanism of DNA cleavage of these complexes was also discussed and proposed as follows: the excited state of Ru(II) polypyridyl complex transfers its energy to the oxygen in solution, producing singlet molecular oxygen as the active oxygen species in the scission reactions, which oxidizes guanine of DNA and leads to cleavage of DNA. These studies are very important to the design of chemical nucleases and DNA-targeting drugs.

Key words: ruthenium(II) polypyridyl complex, DNA, photocleavage, chemical nuclease