化学学报 ›› 2008, Vol. 66 ›› Issue (18): 2059-2064. 上一篇    下一篇

研究论文

羟基磷灰石/丝素蛋白复合纤维的制备及其矿化研究

曹 惠 陈 新 邵正中*

  

  1. (教育部聚合物分子工程重点实验室 复旦大学高分子科学系 先进材料实验室 上海 200433)

  • 收稿日期:2008-03-24 修回日期:2008-05-08 出版日期:2008-09-28 发布日期:2008-09-28
  • 通讯作者: 邵正中

Preparation of Hydroxyapatite/Silk Fibroin Electrospun Fibers and Their Application to Mineralization

CAO, Hui CHEN, Xin SHAO, Zheng-Zhong*   

  1. (Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Department of Macromolecular Science & Advanced Materials Laboratory, Fudan University, Shanghai 200433)
  • Received:2008-03-24 Revised:2008-05-08 Online:2008-09-28 Published:2008-09-28
  • Contact: SHAO, Zheng-Zhong

在以静电纺丝法制备羟基磷灰石(HAP)/丝素蛋白(SF)复合纤维的前提下, 采用同轴共纺法获得了以HAP为“芯”、SF为“皮”的双组分电纺纤维(电纺膜), 并通过扫描和透射电镜、红外光谱以及X射线衍射等手段对电纺纤维进行了表征. 结果表明, HAP均存在于上述两种方法制备的纳米纤维中, 但同轴共纺法不仅可以避免HAP/SF共混电纺时pH值对丝素蛋白结构的影响, 并且能大大提高电纺膜中HAP的含量. 同时, 我们还分别以SF纤维、HAP/SF复合纤维和HAP/SF“皮-芯”纤维作为有机基质, 对羟基磷灰石在其上的矿化过程进行了探索, 结果表明含较多羟基磷灰石的HAP/SF“皮-芯”纤维更有利于矿化的进行.

关键词: 桑蚕丝蛋白, 纳米羟基磷灰石, 同轴共纺, 杂化物

Hydroxyapatite (HAP) (core)/silk fibroin (SF) (shell) nanofiber (mat) was prepared by a co-electrospinning technique based on the successful preparation of HAP/SF composite nanofiber by electrospinning. The results showed that HAP existed in the both fibers, which were characterized by SEM, TEM, FTIR, and XRD. The advantages of co-electrospinning technique exhibited that it solved not only the problem of the influence of pH value on the structure of SF, but also dramatically increased the HAP content in the electrospun mat. In addition, a preliminary study on the mineralization of HAP using the electrospun mat of SF, SF/HAP composite and HAP (core)/SF (shell) mat as organic templates was performed. The results indicated that the HAP (core)/SF (shell) mat with relative high HAP content was more suitable for a mineralization process.

Key words: silk protein, nano-hydroxyapatite (nHAP), co-electrospinning, hybrid