化学学报 ›› 2009, Vol. 0 ›› Issue (1): 88-92. 上一篇    

研究论文

离子液体[BMIM]Cl预处理对微晶纤维素酶解的影响

殷友利李秋瑾苏荣欣齐崴2,何志敏   

  • 收稿日期:2008-08-27 修回日期:2008-10-30 出版日期:2009-01-14 发布日期:2009-12-30
  • 通讯作者: 齐崴 E-mail:qiwei@tju.edu.cn
  • 基金资助:
    国家级.国家自然科学基金

Enzymatic Hydrolysis of Microcrystalline Cellulose with an Ionic Liquid Pretreatment Step

  • Received:2008-08-27 Revised:2008-10-30 Online:2009-01-14 Published:2009-12-30
  • Contact: QI Wei E-mail:qiwei@tju.edu.cn

以微晶纤维素为研究对象,设计了离子液体[BMIM]Cl预处理微晶纤维素Avicel的实验方法以实现纤维素的高效酶解糖化。在[BMIM]Cl中Avicel完全溶解,经水洗沉淀得到再生纤维素,回收后的离子液体可重复利用。预处理后底物酶解的可溶性糖转化率在24h时高达94.65%,较之同样条件下未经预处理底物的酶解糖转化率(48.57%)有飞跃性提升。进一步考察了离子液体预处理对纤维素结构及形态的影响,结果表明:[BMIM]Cl预处理后Avicel氢键减弱;结晶度明显下降,结晶型态由纤维素I型转变为纤维素II型;由规整的平行排布转变为疏松有孔的无序形貌。正是离子液体预处理引起的纤维素微观与宏观结构性质的显著改变使得再生后纤维素酶解的可溶性糖转化率大幅提高。

关键词: 纤维素, 离子液体, 预处理, 酶解

Microcrystalline cellulose Avicel was subjected to an ionic liquid (IL) [BMIM]Cl pretreatment step to obtain a high conversion of soluble sugars in the enzymatic hydrolysis of cellulose. Avicel dissolved completely in [BMIM]Cl, followed by precipitating into deionized water to obtain the regenerated cellulose. The ionic liquid can be recycled for further use. The high performance liquid chromatography (HPLC) analysis results indicated that after 24h, the sugar released form IL-treated Avicel was 94. 65%, much higher than that of the original Avicel (48.57%). Structure, crystallinity and morphology of the IL-treated Avicel were also investigated as following: the hydrogen bonding, detected by FTIR, was decreased; the crystallinity, monitored by XRD, changed significantly from crystalline to amorphous pattern; the morphology, examined by SEM, demonstrated the orderly structure was destroyed remarkably with some pores and holes in the cellulose substrates. These changes of Avicel pretreated with [BMIM]Cl led to the enhancement of sugar conversion during cellulose enzymatic hydrolysis.

Key words: cellulose, ionic liquid, pretreatment, enzymatic hydrolysis