化学学报 ›› 2009, Vol. 67 ›› Issue (4): 318-322. 上一篇    下一篇

研究论文

HPLC-ESI-MS/MS识别蓝莓提取物中的花青素和黄酮醇

袁 帅a 姚胜军a 耿 昱a 蔡 澎b 章文峻b 郭寅龙*,a

  

  1. (a中国科学院上海有机化学研究所质谱中心 上海 200032)
    (b中国科学院上海分院 上海 200031)

  • 收稿日期:2008-03-24 修回日期:2008-08-14 出版日期:2009-02-28 发布日期:2009-02-28
  • 通讯作者: 郭寅龙

Identification of Anthocyanins and Flavonols in Extract of Blueberry by Using HPLC-ESI-MS/MS

Yuan, Shuai a Yao, Shengjun a Geng, Yu a Cai, Peng b
Zhang, Wenjun b Guo, Yinlong *,a
  

  1. (a Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences,
    Shanghai 200032)
    (b Chinese Academy of Sciences Shanghai Branch, Shanghai 200031)
  • Received:2008-03-24 Revised:2008-08-14 Online:2009-02-28 Published:2009-02-28
  • Contact: Guo, Yinlong

用高效液相色谱-电喷雾离子化串联质谱联用技术(HPLC-ESI-MS/MS)对蓝莓提取物中的黄酮类物质进行了分析, 可以快速地鉴定其主要成分为花青素和黄酮醇. 结构上的不同导致花青素和黄酮醇在紫外-可见光谱吸收、离子化以及裂解方式上都存在差异. 尽管花青素和黄酮醇在270 nm处都有吸收, 但两者分别在530和372 nm附近有特征吸收. 负离子模式下, 黄酮醇可以生成负离子和自由基负离子, 而正离子型的花青素则几乎不产生质谱信号, 这一显著差异可用以区别花青素和黄酮醇. 花青素与黄酮醇在串联质谱中分别拥有各自的特征产物离子更进一步证实了其结构存在差别. 该研究对于蓝莓提取物的质量分析与控制很有帮助.

关键词: 花青素, 黄酮醇, 蓝莓, 高效液相色谱-质谱联用, 电喷雾离子化/串联质谱

HPLC-ESI-MS/MS was applied to rapid analysis of flavonoids in the extract of blueberry. Anthocyanins and flavonols were identified as the primary components. The different structures lead to the differences in their UV-Vis absorptions, ionizations and fragmentations. Although both shown absorption maximum at 270 nm, anthocyanins and flavonols possessed characteristic absorption at 530 and 372 nm, respectively. Anthocyanins and flavonols could be distinguished since anion and radical anion were generated from flavonols in the negative ion ESI-MS, while anthocyanins gave no obvious signal under the same conditions. Different characteristic product ions in MS/MS diagram further confirmed the distinctions in structure between anthocyanins and flavonols. This study is helpful for the quality analysis and control of the extract of blueberry.

Key words: anthocyanin, flavonol, blueberry, HPLC-MS, ESI-MS/MS