化学学报 ›› 2010, Vol. 68 ›› Issue (24): 2574-2580. 上一篇    下一篇

研究论文

一种利用同一表面等离子体共振传感器检测多种残留物的方法

张婉洁,苏洋,徐可欣,石婷,刘瑾*   

  1. (天津大学 精密测试技术及仪器国家重点实验室 天津 300072)
  • 收稿日期:2010-03-18 修回日期:2010-06-07 出版日期:2010-12-28 发布日期:2010-08-19
  • 通讯作者: 刘瑾 E-mail:liu_jin@tju.edu.cn
  • 基金资助:

    牛奶中抗生素残留的光学检测方法研究;基于人体组织液透皮抽取和分析的微创血糖检测技术;基于表面等离子体共振的牛奶中抗生素残留的检测方法与应用研究;基于低频超声和表面等离子体共振的微创血糖检测技术;非采血式人体血糖浓度的微创检测技术研究

A Method for the Detection of Various Residues Based on the Same Surface Plasmon Resonance Sensor

ZHANG Wan-Jie, SU Yang, XU Ke-Xin, SHI Ting, LIU Jin   

  1. (State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072)
  • Received:2010-03-18 Revised:2010-06-07 Online:2010-12-28 Published:2010-08-19

提出了一种新的利用基于表面等离子体共振(Surface Plasmon Resonance, SPR)的生物光学传感器检测多种残留物的方法. 引入分子标记技术, 在修饰有羧甲基葡聚糖的芯片表面固定一层抗分子标记物的抗体, 通过对待测样品的两步孵育, 将对多种残留物的检测转化为对同一标记物的测量. 实验采用的分子标记物为牛血清白蛋白(Bovine Serum Albumin, BSA), 分别标记了四种被测物: 卡那霉素、三聚氰胺、氨苄青霉素和链霉素. 对四种被测物的水溶液进行了检测, 检测限分别为50, 10, 1.25和10 ng/mL, 均低于各自的最大残留检出限(Maximum Residue Limit, MRL). 该检测方法解决了原有竞争抑制法在检测时传感器的专一性问题, 实现了传感器的通用性, 同时该方法可推广到其他免疫法检测的领域, 使免疫传感器、免疫试纸等通用性增强.

关键词: 表面等离子体共振, 生物传感器, 抗生素残留, 竞争法, 分子标记

A new method based on surface plasmon resonance (SPR) biosensors for the detection of various residues is proposed. Marking the residues with special moleculars, and the moleculars monoclonal antibody was immobilized on the sensor surface, which is covered with carboxymethyl dextran. So the direct analyte was transformed to general markers after twice incubation of samples. Bovine Serum Albumin (BSA) was used as molecular-marker in the assay, and kanamycin, melamine, ampicillin and streptomycin were marked with BSA separately. Water solution with a series concentration of the antibiotics was detected respectively in this paper. The limits of detection (LODs) reach to 50, 10, 1.25 and 10 ng/mL respectively, being much lower than maximum residue limit (MRL) accordingly. The method has solved the problem that sensors are special for the detection of residues in the competitive assay, and then the sensors will be more available. Furthermore, the method can be applied to other fields of the immunoassay, enhancing the availability of immunesensors and test papers.

Key words: surface plasmon resonance, biosensor, antibiotics residues, competitive assay, molecular mark