Interaction of Three Kinds of Non-steroidal Anti-inflammatory Drugs with DNA Investigated by Two Fluorescence Probes
YANG Chang-Ying1,2; LIU Yi*,1,3; ZENG Fang2; LI Jing2; LI Qiang-Guo1; LI Lin-Wei1
(1 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072) (2 College of Chemistry and Life Science, Three Gorges University, Yichang 443002) (3 State Key Laboratory of Virology, Wuhan 430072)
Abstract：The interaction of three chosen non-steroidal anti-inflammatory drugs (NSAID), indomethacin, sulindac and tolmetin with calf thymus DNA has been investigated by fluorescence with the use of fluorescence probes, ethidium bromide (EB) and neutral red (NR). When bound to DNA, sulindac and indomethacin could quench the fluorescence of EB at low concentration by exchanging with the EB probe, and attained saturation quickly. But sulindac and indomethacin continuously quenched the fluorescence emission and resonance light scattering spectra of NR in the presence of DNA until rather high concentration. These spectral characteristics supported the intercalation of NSAID into DNA as well as electrostatic assembly on the surface of DNA double strands.
杨昌英, 刘义, 曾芳, 李静, 李强国, 李林尉. 两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用[J]. 化学学报, 2007, 65(18): 2076-2080.
YANG Chang-Ying1,2; LIU Yi*,1,3; ZENG Fang2; LI Jing2; LI Qiang-Guo; LI Lin-Wei. Interaction of Three Kinds of Non-steroidal Anti-inflammatory Drugs with DNA Investigated by Two Fluorescence Probes. Acta Chimica Sinica, 2007, 65(18): 2076-2080.