化学学报 ›› 2007, Vol. 65 ›› Issue (18): 2076-2080. 上一篇    下一篇

研究论文

两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用

杨昌英1,2, 刘义*,1,3, 曾芳2, 李静2, 李强国1, 李林尉1   

  1. (1武汉大学化学与分子科学学院 武汉 430072)
    (2三峡大学化学与生命科学学院 宜昌 443002)
    (3病毒学国家重点实验室 武汉 430072)
  • 收稿日期:2006-08-28 修回日期:2007-04-05 出版日期:2007-09-28 发布日期:2007-09-28
  • 通讯作者: 刘义

Interaction of Three Kinds of Non-steroidal Anti-inflammatory Drugs with DNA Investigated by Two Fluorescence Probes

YANG Chang-Ying1,2; LIU Yi*,1,3; ZENG Fang2; LI Jing2; LI Qiang-Guo1; LI Lin-Wei1   

  1. (1 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)
    (2 College of Chemistry and Life Science, Three Gorges University, Yichang 443002)
    (3 State Key Laboratory of Virology, Wuhan 430072)
  • Received:2006-08-28 Revised:2007-04-05 Online:2007-09-28 Published:2007-09-28
  • Contact: LIU Yi

采用溴化乙锭(EB)和中性红(NR)两种荧光探针分别考察了三种非甾体抗炎药(吲哚美辛、舒林酸和托美丁)与DNA的相互作用, 采用荧光发射光谱和共振散射光谱技术得出了基本一致的结论: 吲哚美辛和舒林酸可以在一定程度上与DNA结合(舒林酸更强), 结合方式可能有嵌插作用, 也可能有药物分子在DNA双链表面的组装, 作用力来源于静电作用. 而托美丁几乎不与DNA作用, 因为其疏水性弱, 与DNA分子结合几率小.

关键词: 非甾体抗炎药, DNA, 荧光发射光谱, 共振散射光谱, 探针

The interaction of three chosen non-steroidal anti-inflammatory drugs (NSAID), indomethacin, sulindac and tolmetin with calf thymus DNA has been investigated by fluorescence with the use of fluorescence probes, ethidium bromide (EB) and neutral red (NR). When bound to DNA, sulindac and indomethacin could quench the fluorescence of EB at low concentration by exchanging with the EB probe, and attained saturation quickly. But sulindac and indomethacin continuously quenched the fluorescence emission and resonance light scattering spectra of NR in the presence of DNA until rather high concentration. These spectral characteristics supported the intercalation of NSAID into DNA as well as electrostatic assembly on the surface of DNA double strands.

Key words: non-steroidal anti-inflammatory drug, calf thymus DNA, fluorescence, resonance light scattering spectra, probe