化学学报 ›› 2006, Vol. 64 ›› Issue (9): 953-958. 上一篇    下一篇

研究简报

丁基锡化合物与人血清白蛋白相互作用的研究

张朝红1,董殿波1,臧树良*,2,耿兵1
冯冲1,陈中林1,苏欣1,赵广富2   

  1. (1辽宁大学环境科学系 沈阳 110036)
    (2辽宁大学化学系 沈阳 110036)
  • 收稿日期:2005-09-14 修回日期:2006-01-16 出版日期:2006-05-15 发布日期:2006-05-15
  • 通讯作者: 臧树良

Study on Interaction of Butyltin Compounds withHuman Serum Albumin

ZHANG Zhao-Hong1, DONG Dian-Bo1, ZANG Shu-Liang*,2, GENG Bing1, FENG Chong1, CHEN Zhong-Lin1, SU Xin1, ZHAO Guang-Fu2   

  1. (1 Department of Environmental Science, Liaoning University, Shenyang 110036)
    (2 Department of Chemistry, Liaoning University, Shenyang 110036)
  • Received:2005-09-14 Revised:2006-01-16 Online:2006-05-15 Published:2006-05-15
  • Contact: ZANG Shu-Liang

通过紫外、荧光和CD光谱, 研究了船体防污漆中主要防污成分——三丁基锡(TBT)化合物及其降解产物——一丁基锡(MBT)和二丁基锡(DBT)与人血清白蛋白(HSA)相互作用的方式与程度, 考察了浓度、酸度、有机溶剂和离子强度等因素对相互作用的影响. 结果表明, 丁基锡与HSA的相互作用是双重的, 既有丁基锡中锡离子与HSA的配位作用, 又有丁基锡中丁基基团的疏水作用, 导致HSA二级结构破坏, α-螺旋结构减少和构象转变. 一般情况下, MBT以配位作用为主, TBT以疏水作用为主, DBT两者兼而有之.

关键词: 丁基锡化合物, 人血清白蛋白(HSA), 相互作用

The interaction of tributyltin (TBT), the main components of antifouling paint of ships, and its degradation products of monobutyltin (MBT) and dibutyltin (DBT) with human serum albumin (HSA) was investigated by ultraviolet, fluorescence and circle dichromism spectra. The influences of the concentration, acidity, organic solvent such as ethanol and ionic strength on the interaction of butyltin compounds and HSA molecule were examined to find that the interaction of butyltin compounds and HSA was dual. The butyltin compounds had not only coordinate but also hydrophobic actions with HSA molecule, which led to the destruction of the secondary structure of HSA molecule, decrease of content of α-spiral structure and transformation of conformation. Generally, MBT is inclined to act with HSA molecule through coordination action, while TBT is inclined to do with HSA molecule through hydrophobic action, and DBT can act with HSA molecule adopting both actions synchronously.

Key words: butyltin compound, human serum albumin, interaction