化学学报 ›› 1999, Vol. 57 ›› Issue (10): 1142-1146. 上一篇    下一篇

研究论文

以2, 4, 6-三羟基苯乙酮为基体使用基体辅助激光解吸/电离质 谱法测定核酸分 子的研究

黎军;邓慧敏;赖志辉;赵善楷   

  1. 中山大学测试中心.广州(510275)
  • 出版日期:1999-10-15 发布日期:1999-10-15

A study of using 2, 4, 6-trihydroxyacetophenone as matrix in matrix- assisted laser desorption/ionization mass spectrometry for DNA detection

Li Jun;Deng Huimin;Lai Zhihui;Zhao Shankai   

  1. Zhongshan Univ, Ctr Anal Measur.Guangzhou(510275)
  • Online:1999-10-15 Published:1999-10-15

报道以2,4,6-三羟基苯乙酮(2,4,6-THAP)为基体用基体辅助激光解吸/电离飞行时间质谱法(MALDI-TOF-MS)测定核酸分子的研究。2,4,6-THAP解吸和电离DNA分子的效率很高,测定DNA分子d(T)~1~0的[M-H]^-分辨率可达1130.0,信噪比为366.0,检出限达5×10^-^1^4mol,可以测定相对分子质量高达14800以上混合碱基组成的DNA分子。为提高基体对样品解吸/电离效率,对纯化DNA样品的方法进行了讨论。

关键词: 苯乙酮P, 核酸, 激光, 解吸, 飞行时间质谱法

This paper reports the study on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) of DNA, using 2, 4, 6-trihydroxyacetophenone (2, 4, 6-THAP) as matrix. The results show that 2, 4, 6-THAP has high efficiency in desorbing and ionizing DNA molecules: for [M-H]^- of d(T)~1~0, the resolution is 1130.0, the signal-to-noise ratio is 366.0, and the detection limit is 5×10^-^1^4mol; it can measure mix-based DNA with molecular weight higher than 14800. In order to improve desorption/ionization effects, the purification methods for DNA samples are established.

Key words: ACETOPHENONE P, NUCLEIC ACIDS, LASER, DESORPTION, TIME-OFF FLIGHT MASS SPECTROMETRY

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