|
扩展功能 |
|
| 本文信息 | |
 |
Supporting info |
|
PDF(426KB) |
|
[HTML全文] |
|
参考文献[PDF] |
|
参考文献 |
| 服务与反馈 | |
|
加入引用管理器 |
|
引用本文 |
|
Email Alert |
|
文章反馈 |
|
浏览反馈信息 |
| 本文关键词相关文章 | |
|
碳纳米管 |
|
辣根过氧化酶 |
|
酚 |
|
层层自组装 |
| 本文作者相关文章 | |
|
杨绍明 |
|
黄爱花 |
|
魏志鹏 |
|
江丹 |
|
郑龙珍 |
| PubMed | |
|
Article by Yang, C. M. |
|
Article by Huang, A. H. |
|
Article by Wei, Z. F. |
|
Article by Jiang, D. |
|
Article by Zheng, L. Z. |
将十二烷基硫酸钠（SDS）分散的碳纳米管(CNTs)和辣根过氧化物酶(HRP)通过层层组装方法构筑HRP多层膜酶电极，并将其用于酚类物质的分析检测研究。紫外-可见光谱表明，SDS分散的CNTs可与HRP均匀有效地组装构筑多层酶膜。电化学研究表明，CNTs的引入很好地提高了HRP电极的灵敏度；随着CNTs/HRP组装层数的增加，电极的电化学响应增加。研究结果表明该HRP电极对酚类物质（邻苯二酚、对苯二酚和苯酚）的分析检测具有宽的线性范围、好的灵敏度和抗干扰性。
A horseradish peroxidase (HRP) multilayer enzyme electrode was constructed with carbon
nanotubes (CNT) dispersed by sodium dodecylsulfonate (SDS) and HRP via layer-by-layer (LBL)
self-assembly. UV-Vis spectroscopy and SEM were used to monitor the uniform LBL self-assembly of CNT
and HRP. With catechol as substrate, electrochemical study showed that the use of CNT could obviously
improve the sensitivity of the HRP electrode, and the response of the electrode increased with the increment
of the CNT/HRP bilayer. The performances of the (CNT/HRP)4 electrode were studied for the phenols determination.
The experimental results showed a wide linear range, good sensitivity and anti-interference
ability when the HRP electrode was applied to the determination of phenols (catechol, hydroquinone and
phenol).
1 Basova, E. M.; Ivanov, V. M.; Novikova, K. V. J. Anal.
Chem. 2002, 57, 434.
2 Moskovkin, A. S. J. Anal. Chem. 2002, 57, 507.
3 Rueda, M. E.; Sarabla, L. A.; Herrero, A.; Ortiz, M. C. Anal.
Chim. Acta 2001, 446, 269.
4 Kuban, P.; Flowers, H. Anal. Chim. Acta 2001, 437, 115.
5 Ruzgas, T.; Csöregi, E.; Emnéus, J.; Gorton, L.;
Marko-Varga, G. Anal. Chim. Acta 1996, 330, 123.
6 Korkut, S.; Keskinler, B.; Erhan, E. Talanta 2008, 76, 1147.
7 Cai, C.; Chen, J. Acta Chim. Sinica 2004, 62, 335 (in
Chinese).
(蔡称心, 陈静, 化学学报, 2004, 62, 335.)
8 Iijima, S. Nature 1991, 354, 56.
9 Balasubramanian, K.; Burghard, M. Anal. Bioanal. Chem.
2006, 385, 452.
10 Rivas, G. A.; Rubianes, M. D.; Rodriguez, M. C. Talanta
2007, 74, 291.
11 Chen, D.; Wang, G.; Li, J. J. Phys. Chem. C 2007, 111,
2351.
12 Ovádeková, R.; Jantová, S.; Letašiová, S.; Štepánek, I.;
Labuda, J. Anal. Bioanal. Chem. 2006, 386, 2055.
13 Zhang, J.; Gao, L. Mater. Lett. 2007, 61, 3571.
14 Yan, X. B.; Chen, X. J.; Tay, B. K.; Khor, K. A.
Electrochem. Commun. 2007, 9, 1269.
15 He, L.; Lin, X. F.; Chen, Z. C.; Li, Y. M.; Xu, L. H. Acta
Chim. Sinica 2002, 60, 377 (in Chinese).
(何琳, 林贤福, 陈志春, 李扬眉, 徐立恒, 化学学报,
2002 , 60, 377.)
16 Sha, Y.; Qian, L.; Ma, Y.; Bai, H.; Yang, X. Talanta 2006,
70, 556.
17 Wu, Z.; Feng, W.; Feng, Y.; Liu, Q.; Xu, X.; Sekino, T.;
Fujii, A.; Ozaki, M. Carbon 2007, 45, 1212.
18 Mello, L. D.; Sotomayor, M. D. P. T.; Kubota, L. T. Sens.
Actuators B: Chem. 2003, 96, 636.
19 Santos, A. S.; Pereira, A. C.; Sotomayor, M. D. P. T.;
Tarley, C. R. T.; Durán, N.; Kubota, L. Electroanalysis
2007, 19, 549.