A Fragmentation Study of Kaempferide 3,7-Di-O-glycosides by Electrospray Ionization Tandem Mass Spectrometry
Lu Lin1,3 Shi Ying1,3 Song Fengrui1 Jin Yongri2 Liu Zhiqiang*,1 Liu Shuying1
(1 Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
(2 Jilin University, College of Chemistry, Changchun 130023)
(3 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
Abstract：A serious of new methoxylated flavonol including kaempferide mono-O-glycosides and kaempferide 3,7-di-O-glycosides from Actinidia kolomikta were studied by using electrospray ionization tandem mass spectrometry. The results indicated kaempferide mono-O and di-O have substantial difference in their fragmentation behaviors. Kaempferide mono-O-glycosides yielded abundant aglycone ion ([Y0]－) by loss of glycan residues, however, 3,7-di-O-glycosides mostly generated [ ]－ and [ ]－ ions through removal of glycan residues substituted in C-7 and C-7 respectively, but little aglycone ion from loss of all substituted glycans. Furthermore, the glycosylation site could be easily determined because the [ ]－ and [ ]－ ions shown different fragmentation pathways.
鲁林, 石莹, 宋凤瑞, 金永日, 刘志强, 刘淑莹. 山奈甲黄素-3,7-二-O-多取代黄酮苷的串联质谱研究[J]. 化学学报, 2010, 68(17): 1735-1740.
LU Lin, SHI Ying, SONG Feng-Rui, JIN Yong-Ri, LIU Zhi-Qiang, LIU Shu-Ying. A Fragmentation Study of Kaempferide 3,7-Di-O-glycosides by Electrospray Ionization Tandem Mass Spectrometry. Acta Chimica Sinica, 2010, 68(17): 1735-1740.