化学学报 ›› 2010, Vol. 68 ›› Issue (24): 2600-2608. 上一篇    下一篇

研究论文

新型表面分子印迹法制备苦参碱印迹材料及其分子识别特性

牛庆媛1,高保娇*,1,刘苏宇1,孙中军2   

  1. (1中北大学化学工程系 太原 030051)
    (2陕西嘉禾植物化工有限责任公司 西安 710075)
  • 收稿日期:2009-07-30 修回日期:2009-09-20 出版日期:2010-12-28 发布日期:2010-09-20
  • 通讯作者: 高保娇 E-mail:gaobaojiao@126.com;gaobaojiao@sohu.com
  • 基金资助:

    山西省自然科学基金资助项目(2008021013)

Preparation of Matrine-Imprinted Material Using Novel Surface-Molecular Imprinting Method and Its Molecule Recognition Character

Niu Qingyuan1 Gao Baojiao*,1 Liu Suyu1 Sun Zhongjun2   

  1. (1 Chemical Engineering Department, North University of China, Taiyuan 030051)
    (2 Shaanxi Jiahe Phytochem Co., Ltd. Xi an 710075)
  • Received:2009-07-30 Revised:2009-09-20 Online:2010-12-28 Published:2010-09-20

通过γ-(甲基丙烯酰氧)丙基三甲氧基硅烷的媒介, 将功能大分子聚甲基丙烯酸(PMAA)偶合接枝到硅胶微粒表面, 形成了接枝微粒PMAA/SiO2, 采用本课题组建立的新型分子表面印迹技术, 以生物碱苦参碱(来源于苦豆子)为模板分子, 乙二醇二缩水甘油醚(EGDE)为交联剂, 对接枝在硅胶表面的PMAA大分子链进行了分子印迹, 制备了苦参碱表面分子印迹材料MIP-PMAA/SiO2. 以另一种生物碱金雀花碱(亦来源于苦豆子)为对比物, 采用静态与动态两种方法研究了MIP-PMAA/SiO2对苦参碱的结合性能与分子识别特性. 实验结果表明, 印迹材料MIP-PMAA/SiO2对苦参碱具有特异的识别选择性与优良的结合亲和性, 相对于金雀花碱(与苦参碱共存于苦豆子植物中, 且结构彼此相似), 识别选择性系数为8.14. 此外, 印迹材料MIP-PMAA/SiO2也具有良好的解吸性能, 以含氯化钠的乙酸水溶液作为洗脱液, 17个床体积内解吸率达到99.77%.

关键词: 聚甲基丙烯酸, 硅胶, 分子印迹, 表面印迹, 苦参碱, 分子识别

Polymethylacrylic acid (PMAA) was grafted onto the surface of silica gel particles via the coupling effect of 3-methacryloxypropyl trimethoxysilane (MPS), and the grafting particle PMAA/SiO2 was prepared. Then, the molecular imprinting towards the grafted PMAA was performed with alkaloid matrine which is originated from sophora alopecuroides linn as template molecule and ethylene glycol diglycidyl ether (EGDE) as crosslinking agent by using the novel surface-molecular imprinting technique established by our research group, and the matrine molecule-imprinted material MIP-PMAA/SiO2 was prepared. With another alkaloid, cytisine which is also contained in sophora alopecuroides linn, as a contrast compound, both static and dynamic methods were adopted to study the binding properties and molecule recognition character of MIP-PMAA/SiO2 for matrine. The experiment results show that MIP-PMAA/SiO2 have specific recognition selectivity and excellent binding affinity for matrine. Besides, MIP-PMAA/SiO2 has excellent elution property, with a acetic acid solution containing NaCl as the eluent, the desorption ratios of matrine reach 99.77% in 17 BV.

Key words: polymethylacrylic acid, silica gel, molecular imprinting, surface imprinting, matrine, molecule recognition

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