化学学报 ›› 2011, Vol. 69 ›› Issue (12): 1457-1462. 上一篇    下一篇

研究论文

基于对氨基苯甲酸/硫堇/纳米金共价修饰玻碳电极的葡萄糖生物传感器

牛真真1,于岚岚1,杨冉1,屈凌波*,1,2   

  1. (1郑州大学化学系 郑州 450001)
    (2河南工业大学 郑州 450001)
  • 收稿日期:2010-06-27 修回日期:2010-11-14 出版日期:2011-06-28 发布日期:2011-02-27
  • 通讯作者: 牛真真 E-mail:zhenzhen1012@163.com

Design of a Glucose Biosensor on Glassy Carbon Based on 4-Aminobenzoic Acid/Thionine/Gold Nanoparticles

Niu Zhenzhen1 Yu Lanlan1 Yang Ran1 Qu Lingbo*,1,2   

  1. (1 Department of Chemistry, Zhengzhou University, Zhengzhou 450001)
    (2 Henan University of Industry, Zhengzhou 450001)
  • Received:2010-06-27 Revised:2010-11-14 Online:2011-06-28 Published:2011-02-27

实验制备了以对氨基苯甲酸(4-ABA)、硫堇(TH)、纳米金(Au NPs)共价键合葡萄糖氧化酶的新型葡萄糖生物传感器. 主要采用循环伏安法, 以羟基二茂铁作为电子媒介体, 对含葡萄糖和未含葡萄糖的电解液进行了研究. 结果表明: 传感器的响应电流值随葡萄糖氧化酶膜层数的不同而变化. 考虑到酶电极的长期稳定性与构造简单性, 我们制作了两层葡萄糖氧化酶膜的酶电极. 该传感器对1×10-2 mol/L葡萄糖的响应电流达2.47 μA, 响应时间仅4.7 s. 该生物传感器检测的线性范围为3×10-5~1×10-3 mol/L, 最低检测浓度可达5.8×10-6 mol/L. 该传感器制备简单, 稳定好, 具有一定的使用价值.

关键词: 对氨基苯甲酸, 硫堇, 纳米金, 葡萄糖氧化酶, 羟基二茂铁, 葡萄糖生物传感器

In this study, we construct multilayer films of glucose oxidase/gold nanoparticles on the glassy carbon electrode (GCE) surface using 4-aminobenzoic acid (4-ABA), thionine (TH) and gold nanoparticles (Au NPs) as the covalent attachment cross-linkers. Electrochemical and analytical behavior of the enzyme electrodes was studied by cyclic voltammetry (CV) with hydroxyl ferrocene as mediator in the presence or absence of glucose. The results showed that the current value of the sensor vary with the glucose oxidase layers. In view of the long-term stability of the enzyme electrode and the structural simplicity, we have constructed two layers of glucose oxidase enzyme electrode. When the concentration of glucose solution reached 1×10-2 mol/L, the response current of the sensor would reach 2.47 μA and the response time is only 4.7 s. The bio-sensor linear range of detection is 3×10-5~1×10-3 mol/L, detection limit up to 5.8×10-6 mol/L. The sensor is simple and stable, and the results of the determination of glucose in human serum samples by the biosensor are close to that measured by hospital, so it has some value in use.

Key words: 4-aminobenzoic acid, thionine, gold nanoparticles, glucose oxidase enzyme, hydroxyl ferrocene, glucose biosensor