化学学报 ›› 2011, Vol. 69 ›› Issue (11): 1361-1367. 上一篇    下一篇

研究论文

水溶性荧光共轭聚合物在检测出血热病毒中的应用

陈彦国*,1,2,3,徐保明1,何治柯3,谢卫红4   

  1. (1湖北工业大学化学与环境工程学院 武汉 430068)
    (2湖北师范学院污染物分析与资源化技术湖北省重点实验室 黄石 435002)
    (3武汉大学化学与分子科学学院 武汉 430072)
    (4湖北工业大学生物工程学院 武汉 430068)
  • 收稿日期:2010-10-04 修回日期:2011-01-07 出版日期:2011-06-14 发布日期:2011-01-29
  • 通讯作者: 陈彦国 E-mail:chenyg1965@163.com
  • 基金资助:

    国家自然科学基金项目;国家自然科学基金项目;863计划;湖北省教育厅重点科研基金

Determination of Hantavirus with a Water Soluble Fluorescence Polymer

Chen Yanguo*,1,2,3 Xu Baoming 1 He Zhike3 Xie Weihong 4   

  1. (1 College of Chemistry and Environmental Engineering, Hubei University of Technology, Wuhan 430068)
    (2 Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Hubei Normal University, Huangshi 435002)
    (3College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072)
    (4 College of Biologic Engineering, Hubei University of Technology, Wuhan 430068)
  • Received:2010-10-04 Revised:2011-01-07 Online:2011-06-14 Published:2011-01-29
  • Contact: chen yanguo E-mail:chenyg1965@163.com

利用出血热病毒抗体-L133/抗原-EHF对水溶性荧光共轭聚合物聚(5-甲氧基-2-(3-磺酰化)丙氧基-1,4-对苯撑乙烯), (简写为MPS-PPV)荧光的增强/猝灭作用、抗原-EHF与抗体-L133之间的特异性相互作用及作用前后荧光强度的变化, 建立了一种基于水溶性荧光共轭聚合物荧光猝灭可逆的高灵敏、均相免疫测定新方法, 实现了出血热病毒抗 原-EHF的快速、灵敏检测, 检测抗原-EHF的线性范围是4.8×10-9~5.0×10-8 mol/L, 检出限为1.7×10-9 mol/L. 实验表明, 生物分子对聚合物的荧光增强或猝灭不仅是静电作用, 而且还表现为聚合物形态构造的变化.

关键词: 水溶性荧光共轭聚合物, MPS-PPV, 荧光, 出血热病毒, 生物传感器

The effect of fluorescence enhancing/quenching of the water-soluble fluorescent conjugated polymer poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene] (MPS-PPV) by Hantavirus antibody-L133/antigen-EHF, the specific interaction between the antibody-L133 and its antigen—EHF and the change of fluorescence intensity of MPS-PPV before and after their interaction, a new class of highly sensitive homogenous immunoassay method was set up based on reversible fluorescence quenching in a water-soluble fluorescent conjugated polymer. Hantavirus antigen-EHF was detected with the novel rapid and sensitive method. The linear range and detection limit were 4.8×10-9~5.0×10-8 mol/L and 1.7×10-9 mol/L respectively. It was found that the enhancement or quenching fluorescence intensity of the polymer was not only caused by the electrostatic interaction, but also by the change of the conformation of the polymer as a result of the biomolecules.

Key words: water-soluble fluorescent conjugated polymer, MPS-PPV, fluorescence, hantavirus, biosensor