Abstract：We present a novel core/shell SERS (surface enhanced Raman scattering) probe based on silica-coated gold nanoaggregates and its SERS application in living cells. It is found that the chemical stability and biocompatibility can be greatly improved by coating the gold nanoparticles with a layer of silica. In our experiments, 4MBA (4-mercaptobenzoicacid) is used not only as a Raman reporter, but also an aggregation agent. By coating the gold nanoparticles with the silica shell, the aggregation of gold nanoparticles can be controlled to optimize the SERS activity. TEM images and UV-Vis spectra were employed to investigate the core-shell structure of the probe and the aggregation degree of gold nanoparticles. The results exhibit the strong performance of SERS sensitivity and biocompatibility of the probe in living HeLa and SKBR3 cells, indicating its attraction in further application in intracellular environment.
杨晶, 王著元, 张若虎, 宋春元, 李锦, 高文成, 崔一平. 基于金纳米聚集体的核壳型SERS探针及其细胞内应用[J]. 化学学报, 2011, 69(16): 1890-1894.
YANG Jing, WANG Zhu-Yuan, ZHANG Ruo-Hu, SONG Chun-Yuan, LI Jin, GAO Wen-Cheng, CUI Yi-Ping. Preparation of Silica-coated Aggregated Gold Nanoparticles and Their SERS Performance in Living Cells. Acta Chimica Sinica, 2011, 69(16): 1890-1894.