化学学报 ›› 2011, Vol. 69 ›› Issue (16): 1890-1894. 上一篇    下一篇

研究论文

基于金纳米聚集体的核壳型SERS探针及其细胞内应用

杨晶,王著元,张若虎,宋春元,李锦,高文成,崔一平*   

  1. (东南大学先进光子学中心 南京 210096)
  • 收稿日期:2010-11-14 修回日期:2011-03-11 出版日期:2011-08-28 发布日期:2011-03-30
  • 通讯作者: 崔一平 E-mail:cyp@seu.edu.cn
  • 基金资助:

    复合结构微纳光学探针的光学增强机制及其在生物传感中的应用基础研究;基于表面增强拉曼散射多光谱成像的细胞内药物作用行为表征与研究;基于表面增强拉曼散射的蛋白质微阵列表达谱及成像研究;基于量子点/有机分子复合体系的靶向光敏剂及其光动力疗法机理研究

Preparation of Silica-coated Aggregated Gold Nanoparticles and Their SERS Performance in Living Cells

YANG Jing, WANG Zhu-Yuan, ZHANG Ruo-Hu, SONG Chun-Yuan, LI Jin, GAO Wen-Cheng, CUI Yi-Ping   

  1. (Advanced Photonics Center, Southeast University, Nanjing 210096)
  • Received:2010-11-14 Revised:2011-03-11 Online:2011-08-28 Published:2011-03-30
  • Contact: yiping Cui E-mail:cyp@seu.edu.cn

利用金纳米粒子的聚集体作为表面增强拉曼散射(Surface enhanced Raman scattering, SERS)的增强基底, 合成了一种二氧化硅包裹的核壳型SERS探针, 并成功将该探针应用于活细胞的SERS光谱探测. 实验中利用4-巯基苯甲酸(4-mercaptobenzoicacid, 4MBA)作为拉曼标记物, 并讨论了其在诱导金纳米粒子的聚集过程中的作用. 通过包二氧化硅外壳来实现对金纳米颗粒聚集程度的控制, 保持并优化了该探针的SERS活性. 同时, 通过在金纳米颗粒外包裹一层二氧化硅外壳, 该SERS探针的化学稳定性和生物兼容性得到了很大的提高. 利用紫外-可见吸收光谱仪以及透射电子显微镜研究了该探针中金纳米颗粒的聚集程度以及核壳结构的形貌. 实验结果表明该探针在活细胞内具有很好的SERS灵敏度, 并且生物兼容性好, 可以进一步用于细胞内生理活动监测.

关键词: SERS探针, 聚集金纳米粒子, 二氧化硅外壳, HeLa细胞, SKBR3细胞

We present a novel core/shell SERS (surface enhanced Raman scattering) probe based on silica-coated gold nanoaggregates and its SERS application in living cells. It is found that the chemical stability and biocompatibility can be greatly improved by coating the gold nanoparticles with a layer of silica. In our experiments, 4MBA (4-mercaptobenzoicacid) is used not only as a Raman reporter, but also an aggregation agent. By coating the gold nanoparticles with the silica shell, the aggregation of gold nanoparticles can be controlled to optimize the SERS activity. TEM images and UV-Vis spectra were employed to investigate the core-shell structure of the probe and the aggregation degree of gold nanoparticles. The results exhibit the strong performance of SERS sensitivity and biocompatibility of the probe in living HeLa and SKBR3 cells, indicating its attraction in further application in intracellular environment.

Key words: SERS probe, gold nanoaggregates, silica shell, HeLa cell, SKBR3 cell