a. 湖南大学化学生物传感与计量学国家重点实验室和化学化工学院, 长沙 410082;
b. 中国科学院合肥智能机械研究所, 合肥 230031
Sterically Stabilized,Surface-Enhanced Raman Scattering-Tagged Gold Nanorod Probes for Immunoassay
Lu Junjuna, Guo Hongyanb, Wang Weiyua, Pan Jiangaoa, Hu Jiawena
a. State Key Laboratory for Chemo/Biosensing and Chemometrics and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
b. Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei 230031
Abstract：The immunoassay application of sterically stabilized,surface-enhanced Raman scattering (SERS)-tagged Au nanorod(NR)probes was reported.The probes were prepared by coadsorbing Raman-active molecule 4-mercaptobenzoic acid and biocompatible α-mercapto-ω-carboxyl polyethylene glycol onto the surfaces of Au NRs.The long PEG chain provides both protection and steric stabilization to the probes so that they are able to survive in adverse condition;meanwhile,its terminal carboxyl group binds target entity such as antibody,which imparts detection/recognition functionality to the probes.When the probe recognizes antigen(through the classical"sandwich"structure that was formed due to the highly binding specificity among capture antibody on a solid substrate,detected antigen,and antibody on the probe),the recognition is signaled by the SERS signals of the 4-mercaptobenzoic acid.The detection limit of the Au NR probes,for a single component antigen,was as low as 1×10-9mg·mL-1.