### 介孔碳的快速水相合成及对辣根过氧化物酶的固定

1. 北京工业大学材料科学与工程学院 北京 100124
• 收稿日期:2012-12-30 出版日期:2013-04-14 发布日期:2013-03-08
• 通讯作者: 李群艳 E-mail:qyli@bjut.edu.cn
• 基金资助:

项目受国家自然科学基金(No. 21171014)资助.

### Rapid Aqueous Synthesis of Mesoporous Carbon and Their HRP Immobilization Performance

Sun He, Li Qunyan, Wang Xuewei, Wei Qi, Nie Zuoren

1. College of Materials Science and Engineering, Beijing University of Technology, Beijing 100124
• Received:2012-12-30 Online:2013-04-14 Published:2013-03-08
• Supported by:

Project supported by the National Natural Science Foundation of China (No. 21171014).

Mesoporous carbons have been rapidly synthesized by an aqueous way using polymerization of phloroglucinol and formaldehyde in the presence of triblock copolymer F127 (PEO106PPO70PEO106) under different conditions of hydrochloric acid. The micromorphology, pore structure of the obtained materials were characterized in detail by TEM, SEM and N2 adsorption-desorption. The obtained mesoporous carbons were utilized to adsorb the horseradish peroxidase (HRP) and the thermal stability, pH stability and storage stability of the immobilized HRP activity were also investigated. The results reveal the as-synthesized mesoporous carbon has a wormhole-like mesopore structure. The concentration of hydrochloric acid is a key factor influencing the polymerization rate of phenolic resins. With the concentrations of hydrochloric acid increasing from 0.5 mol/L to 2.5 mol/L, the pore size of mesoporous carbons decreased from 7.6 nm to 5.6 nm, the BET surface areas decreased from 787.8 m2/g to 691.1 m2/g, and pore volumes decreased from 0.64 cm3/g to 0.49 cm3/g. During the process of polymerization, the phase separation appeared in 20 min, while in the reported work, the polymerization of resorcinol and phenol with formaldehyde took much longer time. After incubation at 70 ℃ for 30 min, the activity of immobilized HRP decreased slowly than that of the free HRP. The free and immobilized HRP remained 12.33% and 56.22% of their initial activity, respectively. Compare to free HRP, the immobilized HRP exhibited higher retention activity with a wider pH range between 4.0 and 10.0. The activity of immobilized HRP was also tested for both operation and storage stability. The enzyme activity of immobilized HRP decreased with increasing amount of recycle times. After recycled for 10 d, the activity of immobilized HRP retains 29% of its initial activity. The activity of immobilized enzyme could retain as high as 62% of its initial activity after 50 d of storage at 4 ℃, while the free HRP only retains 31%. The results have shown that the thermal stability, pH stability and storage stability of immobilized enzyme were significantly improved in comparison with free HRP.