Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (06): 789-795.DOI: 10.6023/A1106173 Previous Articles     Next Articles

Special Topic


孔珺a, 邱涵a, 余敏a, 张兵波b   

  1. a 华中科技大学同济医学院附属荆州医院乳腺科 荆州 434100;
    b 同济大学先进材料与纳米生物医学研究院 上海 200092
  • 投稿日期:2011-06-17 修回日期:2011-09-30 发布日期:2011-11-22
  • 通讯作者: 张兵波
  • 基金资助:

    国家自然科学基金(Nos. 51003078, 81171393)、同济大学青年优秀人才培养计划(No. 2009KJ072)和同济大学青年英才计划(优秀青年教师)资助项目.

Effect of Structure on the Fluorescent Properties of the Hydrophilic Modified Quantum Dots

Kong Juna, Qiu Hana, Yu Mina, Zhang Bingbob   

  1. a Jingzhou Hospital Affiliated with Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 434100;
    b The Institute for Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200092
  • Received:2011-06-17 Revised:2011-09-30 Published:2011-11-22
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 51003078, 81171393), Program for Young Excellent Talents in Tongji University (No. 2009KJ072) and Program for Outstanding Young Teachers in Tongji University.

Quantum dots (QDs), as a new class of biological nanoprobe, its fluorescence properties are related to the detection sensitivity. QDs with different core-shell structures vary in the resistance to the fluorescence quenching during surface modification. In this study, a series of core and core-shell QDs with different structure and composition were designed and synthesized. And they were modified with amphiphilic polymer. Their fluorescence properties during the surface modification were monitored by fluorescence spectroscopy. Experimental results show core QDs are the most easily quenched during the surface modification; the ability of resistance to quenching is enhanced after shell coating on core QDs, and this ability increases with the shell layers increase. Results also indicate the structure and composition of shells affect the resistance to fluorescence quenching. The resistance to fluorescence quenching is greatly enhanced when the core-shell QDs has a reasonable shell structure and composition. Furthermore, specific QD nanoprobes are constructed based on selecting appropriate structure of QDs and coupling with folic acid. The prepared QD nanoprobes are used for detection of breast cancer cells on flow cytometry. The results show that by optimizing the structure of core-shell QDs, the modified hydrophilic QDs have good fluorescence property. And the optimized hydrophilic QDs coupled with folic acid have targeting capability of cancer cells.

Key words: quantum dots, hydrophilic modification, fluorescence property, core-shell structure, cell targeting