Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (20): 2127-2132.DOI: 10.6023/A12080499 Previous Articles     Next Articles



王萍, 葛志磊, 裴昊, 王丽华, 樊春海   

  1. 中国科学院上海应用物理研究所物理生物学实验室 上海 201800
  • 投稿日期:2012-08-03 发布日期:2012-09-05
  • 通讯作者: 樊春海
  • 基金资助:

    项目受国家自然科学基金(No. 21075128)和上海市科委(No. 10QA1408200)资助.

Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction

Wang Ping, Ge Zhilei, Pei Hao, Wang Lihua, Fan Chunhai   

  1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800
  • Received:2012-08-03 Published:2012-09-05
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21075128) and Shanghai Municipal Commission for Science and Technology (No. 10QA1408200).

The hybridization chain reaction (HCR), a type of nucleic acid amplification method, was studied by a quartz crystal resonator technique. We probed the properties of the boundary DNA layer with a thickness of the viscous penetration depth at the crystal-solution interface. According to the relationship between the change of resonant frequency and the mass adsorbed on the crystal surface, the kinetics of surface-initiated HCR was measured, as well as the viscosity and the shear modulus of H1/H2 solution with different concentrations near a solid substrate. This work provides a new method for studying nucleic acid reactions at solid surface and mechanistic insight for HCR-based amplification and biodetection.

Key words: hybridization chain reaction, quartz crystal microbalance, DNA, viscosity, interface, penetration depth