Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (03): 339-342.

Communications

Fe3O4@nSiO2@mSiO2磁性介孔微球快速测定溶液中多环芳烃和牛血清蛋白的结合常数

1. 中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室 北京 100085
• 收稿日期:2013-01-17 出版日期:2013-03-14 发布日期:2013-02-04
• 通讯作者: 刘景富 E-mail:jfliu@rcees.ac.cn
• 基金资助:

项目受国家杰出青年科学基金(No.21025729)资助.

Use of Fe3O4@nSiO2@mSiO2 Magnetic Mesoporous Microspheres for Fast Determination of the Sorption Coefficients of Polycyclic Aromatic Hydrocarbons to Bovine Serum Albumin in Aqueous Phase

Pang Long, Liu Jingfu

1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085
• Received:2013-01-17 Online:2013-03-14 Published:2013-02-04
• Supported by:

Project supported by the National Science Fund for Distinguished Young Scholars (No. 21025729).

Polycyclic aromatic hydrocarbons (PAHs) are concerned due to their carcinogenic and teratogenetic effects to human beings. As an important carrier in blood, serum albumin is able to bind to many chemicals and transports them to all parts of the organism. Therefore, it is crucial to determine the partitioning of PAHs to biological phases to evaluate the bioaccumulation of PAHs in organisms. Due to the aqueous solubility of serum albumin, it is difficult to separate the freely dissolved PAHs from the serum albumin associated PAHs with traditional extraction techniques. Although negligible depletion solid-phase microextraction (nd-SPME) is efficient in sensing the freely dissolved PAHs, it suffers from long equilibration time. In this work, we developed a quick approach for the determination of sorption coefficients of PAHs to bovine serum albumin (BSA) by using Fe3O4@nSiO2@mSiO2 magnetic mesoporous microspheres. In the synthesis of Fe3O4@nSiO2@mSiO2 materials, trace amount of hexadecyl trimethyl ammonium (CTAM+) template was kept in the mesopores of these materials to function as sorbent for the extraction of PAHs. The mesoporous structure hinders the extraction of BSA associated PAHs due to the volume exclusion effect, and only the freely dissolved PAHs can access into mesoporous and are extracted by CTAM+. The large surface area (186 m2/g) and high pore volume (0.16 cm3/g) of the Fe3O4@nSiO2@mSiO2 microspheres provide enlarged contact area for extraction. Furthermore, the thin mesoporous layer (≈50 nm) shortened the diffusion distance of PAHs from aqueous phase to CTAM+ sorbent. These above characteristics enhanced the extraction efficiency and reduced the equilibration time (t90%) to 66 min, which is less than 1/200 of that by nd-SPME. In addition, the magnetic microspheres can be conveniently collected from the solution after extraction by applying an appropriate magnetic field. Combining the proposed extraction procedure with GC-MS determination, the partition coefficients of PAHs to BSA were measured with values in the range of 103.94 to 105.00, which were basically consistent with that by nd-SPME (103.53～104.99).