Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (03): 339-342.DOI: 10.6023/A13010089
Pang Long, Liu Jingfu
Project supported by the National Science Fund for Distinguished Young Scholars (No. 21025729).
Polycyclic aromatic hydrocarbons (PAHs) are concerned due to their carcinogenic and teratogenetic effects to human beings. As an important carrier in blood, serum albumin is able to bind to many chemicals and transports them to all parts of the organism. Therefore, it is crucial to determine the partitioning of PAHs to biological phases to evaluate the bioaccumulation of PAHs in organisms. Due to the aqueous solubility of serum albumin, it is difficult to separate the freely dissolved PAHs from the serum albumin associated PAHs with traditional extraction techniques. Although negligible depletion solid-phase microextraction (nd-SPME) is efficient in sensing the freely dissolved PAHs, it suffers from long equilibration time. In this work, we developed a quick approach for the determination of sorption coefficients of PAHs to bovine serum albumin (BSA) by using Fe3O4@nSiO2@mSiO2 magnetic mesoporous microspheres. In the synthesis of Fe3O4@nSiO2@mSiO2 materials, trace amount of hexadecyl trimethyl ammonium (CTAM+) template was kept in the mesopores of these materials to function as sorbent for the extraction of PAHs. The mesoporous structure hinders the extraction of BSA associated PAHs due to the volume exclusion effect, and only the freely dissolved PAHs can access into mesoporous and are extracted by CTAM+. The large surface area (186 m2/g) and high pore volume (0.16 cm3/g) of the Fe3O4@nSiO2@mSiO2 microspheres provide enlarged contact area for extraction. Furthermore, the thin mesoporous layer (&as