Acta Chim. Sinica ›› 2015, Vol. 73 ›› Issue (4): 337-342.DOI: 10.6023/A15010015 Previous Articles     Next Articles



殷薛飞a,b, 刘晓慧a,b, 申华莉a,b, 金红a,b, 杨芃原a,b   

  1. a 复旦大学化学系 上海 200433;
    b 复旦大学生物医学研究院 上海 200032
  • 投稿日期:2015-01-06 发布日期:2015-01-29
  • 通讯作者: 申华莉, 金红;
  • 基金资助:

    项目受863计划(No. 2012AA020201), 国家"211工程"项目高等学校仪器设备和优质资源共享系统(cers-1-66), 高等学校博士学科点专项科研基金(No. 20130071140007)资助.

Whole Proteome of Fusobacterium nucleatum and Quantitative Analysis of Proteomic Change in Cancer Environment

Yin Xuefeia,b, Liu Xiaohuia,b, Shen Hualia,b, Jin Honga,b, Yang Pengyuana,b   

  1. a Department of Chemistry, Fudan University, Shanghai 200433;
    b Institute of Biomedical Science, Fudan University, Shanghai 200032
  • Received:2015-01-06 Published:2015-01-29
  • Supported by:

    Project supported by MOST 863 Program (No. 2012AA020201) and CERS-1-66 (CERS-China Equipment and Education Resources System) and Research Fund for the Doctoral Program of Higher Education of China (No. 20130071140007).

In this paper, we systematically analyzed the proteome of F. nucleatum by using liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) and label free quantification in different culturing pH conditions. We identified over 950 proteins in each LC-MS/MS run. Totally 1198 proteins were identified in two different pH conditions, which correspond to 58% of the protein coding genes among the whole genome of F. nucleatum. The three replicates of pH 6.5 (pH condition of cancer tissue) and pH 7.5 (pH condition of normal tissue) coincide for more than 70% of all the identified peptides and more than 90% of the identified proteins, which indicates the stability and reproducibility of the experiments. By label-free quantitative analysis of the proteome, 1001 proteins have quantitative information. We found that 263 proteins were shown significant expression changed between pH 6.5 and pH 7.5. According to the gene ontology analysis, the significant expression changed proteins were focused on several metabolism processes and functions, such as metal ions transport, thiamin metabolic and carbohydrate metabolism and so on. Butyric acid fermentation is one of the important metabolism pathway in F. nucleatum which also have relationship with colorectal cancer. Butyric acid fermentation pathway including five categories of metabolic enzymes which contains eight protein coding genes. We have found five metabolic enzyme (acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, butyryl-CoA dehydrogenase and acetoacetate:butyrate/acetate CoA transferase) significant down regulation in pH condition of cancer tissue. Therefore, the butyric acid fermentation pathway were inhibited in F. nucleatum when it is living in colorectal cancer micro-environment. As the decrease of the butyric acid production is one of the phenomenon in colorectal cancer patient and F. nucleatum could promote colorectal cancer development, we verified F. nucleatum have contribution to this phenomenon. And the inhibition of butyric acid fermentation may give evidence that F. nucleatum could promote the colorectal cancer development in metabolism level.

Key words: Fusobacterium nucleatum, proteome, label free quantification, pH condition, butyric acid fermentation