Acta Chim. Sinica ›› 2019, Vol. 77 ›› Issue (3): 253-256.DOI: 10.6023/A18100433 Previous Articles     Next Articles

Communication

荧光核酸适配体功能化氧化石墨烯生物传感器用于快速检测氯霉素

卢静荷, 谭淑珍, 朱雨清, 李伟, 陈天啸, 王瑶, 刘陈   

  1. 长沙理工大学 化学与生物工程学院 长沙 410114
  • 收稿日期:2018-10-17 出版日期:2019-03-15 发布日期:2019-01-17
  • 通讯作者: 谭淑珍 E-mail:tsz519@163.com
  • 基金资助:

    项目受湖南省教育厅科研基金(No.17C0033)资助.

Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol

Lu Jinghe, Tan Shuzhen, Zhu Yuqing, Li Wei, Chen Tianxiao, Wang Yao, Liu Chen   

  1. School of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha 410114
  • Received:2018-10-17 Online:2019-03-15 Published:2019-01-17
  • Supported by:

    Project supported by the Scientific Research Fund of Hunan Provincial Education Department (No. 17C0033).

A label free and rapid fluorescent method for quantitative detection of chloramphenicol (CAP) based on graphene oxide (GO) fluorescence functional G-quadruplex probe (FGP) was developed. The FGP consisted of a choramphenicol aptamer and a G-rich sequence. The aptamer was used to bind CAP and the G-quadruplex formed by G-rich sequence was employed as a signal reporter after binding to Thioflavin T (ThT). In the absence of CAP, the FGP was absorbed onto the surface of GO through π-π stacking interactions, which restrained the G-rich sequence to form a G-quadruplex structure. Thus, the fluorescent intensity of background was low. In the addition of the CAP, the aptamer part of FGP could recognize and bind CAP to form a target-FGP complex, which led to the desorption of the complex from GO. Therefore, the free G-rich sequence could form G-quadruplex structure and bind to ThT, resulting a increase in the fluorescence intensity of the solution. We observed that the fluorescence increasement of the sensing platform had a linear relationship with the concentrations of CAP in the range of 2~20 nmol/L, and the limit of detection was 1.45 nmol/L. Besides, this detection system was applied for detecting CAP in the spiked milk, the recovery rate was between 93.2%~103.3%. These results indicated that this developed method can be used to efficiently recognize CAP in real samples.

Key words: aptamer, fluorescence, chloramphenicol, G-quadruplex, graphene oxide