Acta Chimica Sinica ›› 1995, Vol. 53 ›› Issue (4): 362-368. Previous Articles     Next Articles

Original Articles

生物分子电催化的研究 Ⅳ: NADH在亚甲绿修饰石墨电极上的电催化氧化

韩吉林;俞爱民;陈洪渊   

  1. 南京大学化学系
  • 出版日期:1995-04-15 发布日期:1995-04-15

Electrocatalysis of biomolecules at modified electrodes Ⅳ: Electrocatalytic oxidation of reduced nicotinamide adeninedinucleotide at the methylene green modified graphite electrode

HAN JILIN;YU AIMIN;CHEN HONGYUAN   

  • Online:1995-04-15 Published:1995-04-15

The chemically modified electrode(CME), which was constructed by adsorbing the mediator methylene green(MG) onto the spectrographic graphite electrode, shows significant electrocatalytic activity to the oxidation of reduced nicotinamide adeninedinucleotide (NADH), reducing oxidation overpotential by 500mV. In the potential range from -0.25V to +0.1V the adsorbed mediator MG behaves as one-electron reversible redox process involving one proton. In the medium of 0.1mol/dm^3 phosphate buffer (pH=7.0) the surface formal potential E°' and apparent electron-transfer rate of the adsorbed mediator MG were found to be -0.14V and 4.4s^-^1, respectively. The CME was tested as a electrochemical NADH detector, which shows high sensitivity and good stability. The CME was also characterized by ESCA and ATR.

Key words: NICOTINAMIDE ADENINE DINUCLEOTIDE, MODIFIED ELECTRODE, METHYLENE HALIDE

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