Acta Chimica Sinica ›› 1995, Vol. 53 ›› Issue (9): 883-888. Previous Articles     Next Articles

Original Articles

用桑蚕丝素蛋白固定脲酶的研究及脲酶电极的制备

邵正中;方跃;邓家祺;于同隐   

  1. 复旦大学高分子科学系;华东理工大学分析测试中心;复旦大学化学系;复旦大学高分子科学系
  • 出版日期:1995-09-15 发布日期:1995-09-15

Bombyx mori silk fibroin material for immobilization of urease and preparation of urea electrode

SHAO ZHENGZHONG;FANG YUE;DENG JIAQI;YU TONGYIN   

  • Online:1995-09-15 Published:1995-09-15

A novel method for enzyme immobilization was developed, which is based on the structural transition of the silk fibroin membrane from random coil or α-helix to β-sheet by using methanol, the enzyme was immobilized in the spaces formed by the intermolecular hydrogen bonds in the β-sheet conformation. The resulting urease electrode performs satisfactorily under appropriate operation conditions. It exhibits excellent thermal stability and over three months useful lifetime. In principle, this method should be applicable to immobilize other soluble enzymes which do not destroy the molecular structure of silk fibroin.

Key words: METHANOL, ENZYME ELECTRODES, RAMAN SPECTROMETRY, UREASE

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