Acta Chimica Sinica ›› 1995, Vol. 53 ›› Issue (9): 889-894. Previous Articles     Next Articles

Original Articles

基体辅助激光解吸质谱法测定蛋白质分子量

钟峰;赵善楷   

  1. 中山大学测试中心
  • 出版日期:1995-09-15 发布日期:1995-09-15

Matrix-assisted laser desorption mass spectrometry of proteins

ZHONG FENG;ZHAO SHANKAI   

  • Online:1995-09-15 Published:1995-09-15

By the use of a home-built research-grade laser time-of-flight mass spectrometer and with a new recently developed new method, matrix-assisted laser desorption ionization (MALDI), the molecular weight of proteins, such as lysozyme, cytochrome C, myoglobin, trypsin, protease and albumin as well as mixtures of proteins are determined. The results demonstrated that with the new method molecular weight of proteins can be determined fastly (witin ten minutes), accurately (absolute error=±0.1%) and sensitively (10^-^1^2~10^-^1^5mol), that is far superior than those traditional methods.

Key words: MOLECULAR WEIGHT, PROTEASE, CYTOCHROME, TRYPSIN, ALBUMINE, PROTEIN, LYSOZYME, MYOGLOBIN, TIME-OFF FLIGHT MASS SPECTROMETRY

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