Acta Chimica Sinica ›› 1996, Vol. 54 ›› Issue (4): 392-397. Previous Articles     Next Articles

Original Articles

利用超载洗脱技术在TSK Gel CM-5PW分析柱上进行溶菌酶百毫克级制备

唐晋滨;黄骏雄   

  1. 中国科学院生态环境研究中心
  • 出版日期:1996-04-15 发布日期:1996-04-15

Preparative separation of lysozyme on TSK Gel CM-5PW analyticial column on the scale of several hundred milligrams by overloading elution technique

TANG JINBIN;HUANG JUNXIONG   

  • Online:1996-04-15 Published:1996-04-15

The separation and purification of lysozyme from 150mg protein mixture on TSK Gel CM-5PW, an analytical cation exchange column (Φ7.5 ×75mm), by overloading elution mode of nonlinear chromatography was reported in this paper. It has been found that more than 90% of recovery and a satisfactory purity of lysozyme fraction, which was checked with standard sample by high performance capillary electrophoresis and UV spectrophotometry, was achieved.

Key words: SEPARATION, LYSOZYME, PURIFICATION

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