Acta Chimica Sinica ›› 1998, Vol. 56 ›› Issue (1): 87-92. Previous Articles     Next Articles

Original Articles

毛细管区带电泳对手性药物盐酸美西律和盐酸异博定的对映体分离

王志;黄爱今;孙亦梁;孙曾培   

  1. 北京大学化学系;中国药品生物制品检定所
  • 出版日期:1998-01-15 发布日期:1998-01-15

Enantiomeric separation of mexiletine and verapamil by cyclodextrin-mediated capillary zone electrophoresis

WANG ZHI;HUANG AIJIN;SUN YILIANG;SUN ZENGPEI   

  • Online:1998-01-15 Published:1998-01-15

The enantiomeric separation of chiral drugs mexiletine and verapamil was investigated using cyclodextrins added buffers at acidic conditions in capillary zone electrophoresis. The results show that the type and concentration of chiral selector, buffer concentration etc, influence the separation. Among the five cyclodextrins (CDs) investigated, i.e., α-CD, β-CD, Heptakis(2, 6-di-O-methyl)-β-CD (DM-β-CD), Hydroxypropyl-β-CD(HP-β -CD) and γ-CD, HP-β-CD gave the best separation of verapamil and mexiletine is at 9 and 30 mmol/L, respectively and the optimal Tris-H3PO4 (pH 2.3) buffer concentration at 100 mmol/L. The separation was improved by the addition of 0.05% hydroxypropyl cellulose (HPC) to the buffer. Under the optimized separation conditions, near baseline enantiomeric separation was achieved for mexiletine and enantiomers of verapamil were also fairly well separated.

Key words: SEPARATION, CYCLODEXTRIN, ENANTIOMORPH, CAPILLARY ZONE ELECTROPHORESIS

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