Acta Chimica Sinica ›› 2001, Vol. 59 ›› Issue (4): 615-618. Previous Articles     Next Articles

Original Articles

电喷雾离子化飞行时间质谱研究鸡蛋清溶菌酶与β-环糊精 的新型复合物

余 中天;郭寅龙;张尊建;相秉仁;安登魁   

  1. 中国科学院上海有机化学研究所.上海(200032);中国药科大学分析计算中心.南 京(210009)
  • 出版日期:2001-04-15 发布日期:2001-04-15

Study on the complexes of egg-white lysozyme and β- cyclodextrin by electrospray ionization time-of-flight mass spectrometry

Yu zhongtian;Guo Yinlong;Zhang Zunjian;Xiang Bingren;An Dengkui   

  1. Shanghai Inst Organ Chem., CAS.Shanghai(200032);China Pharmaceut Univ., Anal & Comput Center.Nanjing(210009)
  • Online:2001-04-15 Published:2001-04-15

Electrospray ionization time-of-flight mass spectrometry is a good tool for the characterization of noncovalent complexes because of "soft" ionization and condition. Under our experimental conditions. the nonspecific noncovalent interactions of egg-white lysozyme with β -cyclodextrin have been observed by electrospray ionization/time- of flight mass spectrometry. Some mass spectrometic feature, such as ion abundance enhancement by selecting appropriate nozzle potential, and stoichimetries, show that the noncovalent complexes between lysozyme and β-cyclodextrin have been formed at a ratio of 1:1, 1:2, 1:3 in higher concentration and almost left ratio 1:1 in lower concentration of β-cyclodextrin. These results imply that the complexes should be nonspecific complexes. In addition, we founded that the appropriate condition, such as addition of some organic solvent of suitable SCIEX chamber temperature, is conductive to the ionization of noncovalent conplexes.

Key words: TIME-OFF FLIGHT MASS SPECTROMETRY, LYSOZYME, CYCLODEXTRIN, COMPLEX

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