Acta Chimica Sinica ›› 2001, Vol. 59 ›› Issue (5): 769-773. Previous Articles     Next Articles

Original Articles

苏云金芽胞杆菌含不同质粒和不同基因工程菌的生长代谢

林晓燕;刘义;孙明;高振霆;屈松生;喻子牛   

  1. 华中农业大学微生物科学技术系;农业部农业微生物重点开放实验室;武汉大学 化学系.武汉(430072)
  • 出版日期:2001-05-15 发布日期:2001-05-15

Thermokinetic change of several bacillus thuringiensis strains including different plasmids or having different genes as studied by microcalorimetry

Lin Xiaoyan;Liu Yi;Sun Ming;Gao Zhenting;Qu Songsheng;Yu Ziniu   

  1. Wuhan Univ, Dept Chem.Wuhan(430072)
  • Online:2001-05-15 Published:2001-05-15

The heat out -put of metablism of different bacillus thuringiensis has been studied by means of microcalorimetry. The experimental strains were a wild type (YBT-1463) and engineering type (BMB160, bmb171, BMB-304-171-A, BMB-304-171-B). YBT-1463 contains fourteen plasmids, BMB160 contains six plasmids but no insecticidal crystal protein. BMB171 have neither plasmids nor insectividal crystal protein gene. BMB-304-171 -A and BMB-304-171- B each havew a insecticidal crystak protein gene , one is the gene of cryⅠ Ac the other is ctyⅠC. The experimental data show that the heat out -put of metabolism of BMB171 is much more than that of YBT-1463 and BMB160, and the metabolic heats of BMB-304-171-A and BMB-304-171-B are also less than that BMB171. These results indicate that the endogenuous plasmids have great effect on the metabolism of Bacillus thuringiensis. It is the same for the genes which are tranformed into BMB171. sime there is no much difference between the genes of cryⅠAc and ctyⅠC, there is no dramatic change in thermokinetism. The first report of the thermokinetism in Bacillus thuringensis is benficial to the control of fermention .

Key words: MICROCALORIMETRIC METHOD, INSECTICIDES, NGINEERED BACTERIA

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