Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (1): 105-108. Previous Articles     Next Articles

Original Articles

光谱法研究Ge-132与DNA的作用机理

李文友;朱守田;何锡文;梁宏   

  1. 南开大学化学系;广西师范大学化学与生命科学学院
  • 出版日期:2002-01-15 发布日期:2002-01-15

Study on the interaction mechanism between Ge-132 and DNA by spectral methods

LI WENYOU;ZHU SHOUTIAN;HE XIWEN;LIANG HONG   

  • Online:2002-01-15 Published:2002-01-15

The interaction between Ge-132 and DNA was investigated using absorption spectra, DNA alkaline denaturation and fluorescence spectra. Based on the measurements of A~2~6~0 in different pH media, the alkaline denaturation curves of CT DNA in the absence of and in the presence of Ge-132 were obtained. The results showed that the alkaline denaturation for Ge-132-DNA complex occurred at a higher pH than for DNA alone and the hyperchromicity of Ge-132-DNA complex was smaller than that of DNA, which suggested the intercalation mechanism of Ge-132 into DNA bases. All the exprimental results indicated that the intercalative binding was the major mode for the interaction of Ge-132 with DNA.

Key words: ABSORPTION SPECTROMETRY, DEOXYRIBONUCLEIC ACID, ORGANO GERMANIUM, INTERCALATION REACTION, ANTICARCINOGEN

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