Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (2): 377-381. Previous Articles     Next Articles

Original Articles

α-淀粉酶在阴离子化PET表面的自组装膜及其AFM研究

何琳;林贤福;陈志春;李扬眉;徐立恒   

  1. 浙江大学化学系.杭州(310027)
  • 出版日期:2002-02-15 发布日期:2002-02-15

Investigation of α-amylase self-assembled films on the negatively charged PET surface by AFM

He Lin;Lin Xianfu;Chen Zhichun;Li Yangmei;Xu Liheng   

  1. Zhejiang Univ, Dept Chem.Hangzhou(310027)
  • Online:2002-02-15 Published:2002-02-15

It is of great interest to prepare artificial architecture of protein in thin film, which has been the focus of bioengineerign in recent years. The self-assembled films of α-amylase on the negatively charged PET(PET-CO^-~2) is fabricated and the AFM results are described in this paper. The surface of PET-CO^-~2 was covered by high-density layer of α-amylase, whose thickness was 5~10 nm. The molecules of α-amylase aggregated in the layer which was obtained from the pH 6.0 solution, and the shape of molecules became shaper, which resulted in lagr RMS. On the lower-charged substrate (PET-CO^- ~2), the aggregating molecules could be found. With raising the charge density of substrate the shape of molecules became sharper.

Key words: AMYLASE, MONOMOLECULAR FILMS, PET, POLYETHYLENE TEREPHTHALATE

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