Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (3): 393-397. Previous Articles     Next Articles

Original Articles

超细银-金复合颗粒增强酶生物传感器的研究

任汀菱;唐芳琼   

  1. 中国科学院理化技术研究所.北京(100101)
  • 出版日期:2002-03-15 发布日期:2002-03-15

Enhancement Effect ofAg-Au Nanoparticles on Glucose Biosensor Sensitivity

Ren Xiangling;Tang Fangqiong   

  1. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences.Beijing(100101)
  • Online:2002-03-15 Published:2002-03-15

Hydrophobic Ag-Su nanoparticles are produced in AOT [Aeroslo OT, sodium bis (2-ethyhexyl) sulfosuccinate]-cyclohexane reverse micellar system. Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix, which is composed of hydrophobic Ag-Au particles and polyvinyl butyral (PVB) by a sol-gel method. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilized enzyme. The current response is increased from tens of nanoamperometer (nA) to ten thousands of nanoamperometer to the same glucose concentration. The experiment and theory proved the function of nanoparticles in immobilization of enzyme. The catalysis of enzyme improved by nanoparticles was discussed. This research provides experimental and theoretical basis for preparation of useful glucose biosensor.

Key words: SILVER, GOLD, NANOPHASE MATERIALS, FIXED ENZYME, SENSORS, GLUCOSE OXIDASE

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