Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (3): 504-508. Previous Articles     Next Articles

Original Articles

大孔聚甲基丙烯酸缩水甘油酯固定化酶载体的合成及性能研究

乌云高娃;卢冠忠;郭杨龙;王筠松;魏东芝   

  1. 华东理工大学工业催化研究所.上海(200237);生物反应器国家重点实验室.上 海(200237)
  • 出版日期:2002-03-15 发布日期:2002-03-15

The Studies on Synthesis and Properties of Glycidyl Methacrylate-N, N'-methylene-bis (acrylamide) Copolymer Supports of Enzyme Catalyst

Wuyun Gaowa;Lu Guanzhong;Guo Yanglong;Wang Yunsong;Wei Dongzhi   

  1. Research Institute of Industrial Catalysis East China University of Science and Technology.Shanghai(200237);East China University of Science and Technology.Shanghai(200237)
  • Online:2002-03-15 Published:2002-03-15

Reactive, macroporous and beaded glycidyl methacrylate-N, N'- methylene-bis (acrylamide) copolymers of widely varying crosslink densities were synthesized with formamide as progenic agent by inverse suspension copolymerization. The effects of crosslink density on structure properties and apparent activity of immobilized penicillin G acylase were investigated.Specific surface area increased, pore volume, porosity and average pore diameter decreased with concomitant increase in crosslinking monomer. Immobilized enzyme activity of 254.8 U/g was obtained with a polymer with ω = 60% crosslinking having a mean pore diameter of 4.9 nm and an oxirane group content of 0.005 mmol·m~(-2).

Key words: METHYLPROPENOIC ACID ESTER, GLYCIDYL ESTER, PROPENAMIDE, SUSPENSION POLYMERIZATION, PENICILLINASE

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