Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (9): 1677-1681. Previous Articles     Next Articles

Original Articles

毛细管电泳对脑外伤脑脊髓液中兴奋性氨基酸的测定

陆豪杰;郭寅龙;梁冰;欧庆瑜   

  1. 中国科学院上海有机化学研究所.上海(200032);中国科学院兰州化学物理研 究所,兰州(730000)
  • 出版日期:2002-09-15 发布日期:2002-09-15

Determination of Excitatory Amino Acids in Cerebrospinal Fluid Following Cerebral Injury by Capillary Electrophoresis

Lu Haojie;Guo Yinlong;Liang Bing;Ou Qingyu   

  1. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences.Shanghai(200032);Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou(730000)
  • Online:2002-09-15 Published:2002-09-15

Capillary electrophoresis was developed to determine excitatory amino acids in cerebrospinal fluid following cerebral injury. The effect of injection module, concentration of buffer, organic additive, and derivative conditions on determination minute quantity of excitatory amino acids in cerebrospinal fluid was investigated. The limitation of detection was less than 1.7 * 10~(-7)mol/L under the optimum conditions with benzene carboxylic acid as an internal standard. The reproducibility was also satisfactory with the relative standard deviation less than 2%.

Key words: ELECTROPHORESIS, AMINO ACID, CEREBRO-SPINAL FLUID

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