Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (9): 1727-1731. Previous Articles     Next Articles

Original Articles

基于美洲蜚蠊下颚须的整合受体生物传感器测定抗坏血酸

洪丽;尹屹梅;郑赛晶;段亚楠;林祥钦   

  1. 中国科学技术大学化学系,合肥(230026)
  • 出版日期:2002-09-15 发布日期:2002-09-15

Intact-Chemoreceptor-Based Biosensor: Detection of Ascorbic Acid

Hong Li;Yin Yimei;Zheng Saijing;Duan Yanan;Lin Xiangqin   

  1. Department of Chemistry, University of Science and Technology of China,Hefei(230026)
  • Online:2002-09-15 Published:2002-09-15

An intact-chemoreceptor-based biosensor based on the gustatory organ, the maxillary palp of Periplaneta americana, is reported, in which the maxillary palp serves as not only the molecular recognition elements but also the biological transducers. The binding events between ligand and chemoreceptor induce action potentials (APs) whose frequency correlates directly to the concentration of stimulants. The APs were recorded with a three-electrode system and collected by sound card method developed by our group. This is the first time that the biosensor is reported to yield quantitative linear dose-response data over the concentration range from 5 * 10~(-5) to 5 * 10~(-2) mol/L. The new biosensor possesses some advantages over the biosensor based on isolated receptor: shorter response time (<1 s), higher degrees of sensitivity (detect-limitation below 10~(-6) mol/L), broader dynamic response range (over four orders of magnitude) and more preferable reproducibility.

Key words: BIOSENSORS, VITAMINC

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