Acta Chimica Sinica ›› 2003, Vol. 61 ›› Issue (10): 1682-1685. Previous Articles     Next Articles

Original Articles

基于硫化镉纳米团簇标记DNA电化学传感的研究

祝宁宁;张爱平;何品刚;方禹之   

  1. 华东师范大学化学系
  • 出版日期:2003-10-15 发布日期:2003-10-15

Cadmium Sulfide Nanocluster-based Electrochemical Sensing ofDNA Hybridization

Zhu Ningning;Zhang Aiping;He Pingang;Fang Yuzhi   

  1. Department of Chemistry, East China Normal University
  • Online:2003-10-15 Published:2003-10-15

Synthesis of cadmium sulfide ( CdS) nanoclusters and its application in an electrochemical DNA hybridization detection were described. The nanoclusters were successfully labeled to a 5'-amino group capped oligonucleotides probe that is related to the colitoxin gene. The DNA genetic sensing assay relies on the target oligonucleotides on the glassy carbon electrode (GCE) hybridized with the CdS nanocluster-oligonucleotides DNA probe. Hybridization events between the probe aid target were monitored by the release of the CdS anchored on the hybrids by oxidative dissolution and the indirect determination of the solubilized Cd~(2+) ions by sensitive anodic stripping voltammetry (ASV) . The detection limit is 10 pmol/L of the complementary target oligonucleotides.

Key words: CADMIUM SULFIDE, NANOPHASE MATERIALS, OLIGONUCLEOTIDE, VOLTAMMETRY, ESCHERICHIA COLI, ELECTROCHEMISTRY

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