Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (1): 66-70. Previous Articles     Next Articles

毛细管电泳紫外检测条件下单碱基分离DNA片段

王前, 许旭   

  1. 中国科学院上海有机化学研究所分析测试中心, 上海, 200032
  • 收稿日期:2003-05-26 修回日期:2003-09-05 出版日期:2004-01-14 发布日期:2014-01-26
  • 通讯作者: 许旭 E-mail::E-mail:xuxu@mail.sioc.ac.cn.
  • 基金资助:
    中国科学院创新工程重大项目基金(No.KSCX1-06)资助.

DNA Separation as Single-base-pair Resolution by Capillary Electrophoresis with Ultraviolet Detector

WANG Qian, XU Xu   

  1. Analytical and Testing Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
  • Received:2003-05-26 Revised:2003-09-05 Online:2004-01-14 Published:2014-01-26

The water-soluble mixtures of polymers, poly(N,N-dimethylacrylamide) (PDMA)/polyvinylpyrrolidone (PVP), were synthesized and used in capillary electrophoresis (CE) for DNA separation. With the optimal concentration of PDMA/PVP polymer solution, the doublet of 123/124 bp in pBR322/Hae Ⅲ Markers could be separated with UV detector and the resolution value was 0.72. When the denaturant formamide (φ≥0.10) was added in the polymer solution, DNA fragments of 123/124 could be also separated well with the resolution as 0.75, which quite met the requirement of DNA sequencing (Rs>0.59). In addition, the lower viscosity of the polymer solution made the practice easier. The sample stacking effect was validated in the experiments. The medium showed single-base-pair DNA separation by capillary electrophoresis even with ultraviolet detector and uncoated capillary column.

Key words: capillary electrophoresis, non-gel sieving, synthetic polymer, DNA separation, UV detector, single-base-pair separation