Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (1): 71-77. Previous Articles     Next Articles

微板式磁化学发光酶免疫分析法对人绒毛膜促性腺激素(HCG)的灵敏快速测定

赵利霞, 林金明, 屈锋   

  1. 中国科学院生态环境研究中心, 北京, 100085
  • 收稿日期:2003-05-07 修回日期:2003-08-31 出版日期:2004-01-14 发布日期:2014-01-26
  • 通讯作者: 林金明 E-mail::E-mail:jmlin@mail.rcees.ac.cn
  • 基金资助:
    国家杰出青年科学基金(No.20125514)资助项目.

Micro-plate Magnetic Chemiluminescence Enzyme Immunoassay for Rapid, Sensitive Determination of Human Chorionic Gonadotropin (HCG)

ZHAO Li-Xia, LIN Jin-Ming, QU Feng   

  1. Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085
  • Received:2003-05-07 Revised:2003-08-31 Online:2004-01-14 Published:2014-01-26

A micro-plate magnetic chemiluminescence enzyme immunoassay for the determination of human chorionic gonadotropin (HCG) based on the improvement in the method of the magnetic immunoenzyme spectrophotometric assay was established. The enzyme alkaline phosphatase (ALP) was used as labeled reagent, which reacted with the chemiluminescence reagent of 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) in buffer solution. The sensitivity of the present chemiluminescent method was 13 times higher than that of spectrophotometry. The linear range was 0.15~500 mIU/mL HCG; the within run and between run CVs were both below 18%. This method was applied to detect HCG in serum and the percent recovery was in the range of 80%~116%. Compared with other chemiluminescence immunoassays, the correlation coefficient was 0.965. It was firstly reported to use enzyme immunoassay for the detection of HCG in saliva, which will break a new way for establishing a non-invasive method.

Key words: chemiluminescence, human chorionic gonadotropin(HCG), enzyme immunoassay, saliva