Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (11): 997-1002. Previous Articles     Next Articles

Original Articles

金纳米微粒作探针共振瑞利散射光谱法测定卡那霉素

何佑秋,刘绍璞*,刘忠芳,胡小莉,鲁群岷   

  1. (西南师范大学化学化工学院 重庆 400715)
  • 收稿日期:2004-09-06 修回日期:2005-02-20 出版日期:2005-06-14 发布日期:2010-12-10
  • 通讯作者: 刘绍璞

Resonance Rayleigh Scattering Spectral Method for the Determination of Kanamycin with Gold Nanoparticle as Probe

HE You-Qiu, LIU Shao-Pu*, LIU Zhong-Fang, HU Xiao-Li, LU Qun-Min   

  1. (School of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715)
  • Received:2004-09-06 Revised:2005-02-20 Online:2005-06-14 Published:2010-12-10
  • Contact: LIU Shao-Pu

In a citrate containing solution, anion of citrate self-assembled on Au nanoparticle surface with positive charge, making Au nanoparticle become a supermolecular compound with negative charge surrounded by citrate. In a weak acid medium of pH 4.4~6.8, the supermolecular compound can combine with kanamycin by the virtue of electrostatic and hydrophobic interaction, forming aggregate with bigger diameters (average diameter increased from 12 to 20 nm ). The aggregate made plasmon absorption band of glod nanoparticle show evident bathochromic effect (Δλ=102 nm), resonance Rayleigh scattering (RRS) in- tensity be enhanced greatly and the resonance nonlinear scattering such as frequency double scattering (FDS) and second order scattering (SOS) be increased markedly as well. The maximum scattering peaks are at 280 nm for RRS, 310 nm for FDS and 480 nm for SOS respectively. In optimum conditions, there was a linear relationship between scattering intensity ΔI and the concentration of kanamycin, and the highest sensitivity was RRS. So gold nanoparticle could be the RRS probe of high sensitivity for kanamycin with the detection limits of kanamycin being 10.52 ng•mL-1. The method has good selectivity and has been successfully applied to the detection of kanamycin in clinic serum samples. In addition, the reaction mechanism and the reasons for the enhancement of RRS were discussed.

Key words: gold nanoparticle, resonance Rayleigh scattering spectrum, kanamycin