Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (17): 1847-1853. Previous Articles     Next Articles

Original Articles

囊泡表面分子间通讯交流体系的构建: 利用受体的分子识别行为调控酶的活性

田文杰*,1; 神前健嗣2; 佐佐木善浩2; 岳秀丽3; 金黎明1; 范圣第1; 菊池纯一*,2   

  1. (1大连民族学院生命科学学院 大连 116600)
    (2 日本奈良先端科学技术大学院大学物质创成科学研究科 奈良 630-0192)
    (3 哈尔滨工业大学市政环境工程学院 哈尔滨 150001)
  • 收稿日期:2006-04-03 修回日期:2006-07-24 出版日期:2006-09-14 发布日期:2006-09-14
  • 通讯作者: 田文杰

Intermolecular Communication on Lipid Bilayer Membrane: Molecular Recognition Behavior in the Receptor Site to Control Enzymatic Activity

TIAN Wen-Jie*,1; KOZAKI Kenji2; SASAKI Yoshihiro2; YUE Xiu-Li3; JIN Li-Ming1; FAN Sheng-Di1; KIKUCHI Jun-ichi*,2   

  1. (1 College of Life Sicence, Dalian Nationalities University, Dalian 116600, China)
    (2 Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, 630-0192, Japan)
    (3 School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150001, China)
  • Received:2006-04-03 Revised:2006-07-24 Online:2006-09-14 Published:2006-09-14
  • Contact: TIAN Wen-Jie

Functionalized lipid bilayer vesicles on which molecular recognition by an artificial receptor synchronized with catalytic reaction by an enzyme were prepared in aqueous media. The system was constructed with the following molecular components: an alkyl amine as a receptor, a pyridoxal derivative as a signal ligand to the receptor, lactate dehydrogenase as an enzyme, Cu2+ ions as a mediator between the receptor and the enzyme, and a synthetic peptide lipid as a membrane-forming amphiphile. The design of the present supramolecular assembly was inspired by biological signal transduction system on the cell membrane. Molecular recognition behavior of the signal ligand by the receptor was well correlated with the hydrophobic parameter of the receptor molecules. Marked signal specificity through electrostatic interactions between the signal and the membrane surface was also observed. Strong binding of the mediator species toward the signal-receptor complex in 1∶2 stoichiometry was clarified. As a result, the enzymatic activity on the membrane surface was effectively controlled through molecular recognition by a hydrophobic alkyl amine as the receptor toward pyridoxal 5'-phosphate as the signal ligand.

Key words: intermolecular communication, molecular recognition, lipid bilayer vesicle, receptor, enzyme