Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (22): 2259-2264. Previous Articles     Next Articles

Original Articles

DNA-纳米多孔羟基磷灰石修饰电极用于研究DNA与亚甲基蓝的相互作用

王桂香1,2, 潘芊秀1, 张京京1, 王怀生*,1   

  1. (1聊城大学化学化工学院 分析化学研究所 聊城 252059)
    (2泰山学院材料与化学工程系 泰安 271021)
  • 收稿日期:2006-01-05 修回日期:2006-04-28 出版日期:2006-11-28 发布日期:2006-11-28
  • 通讯作者: 王怀生

DNA-Nano-porous Hydroxyapatite Modified Electrode Applied to the Study on the Interactions of DNA with Methylene Blue

WANG Gui-Xiang1,2; PAN Qian-Xiu1; ZHANG Jing-Jing1; WANG Huai-Sheng*,1   

  1. (1 Department of Chemistry, Institute of Analytical Chemistry, Liaocheng University, Liaocheng 252059)
    (2 Department of Materials Science and Chemical Engineering, Taishan University, Tai'an 271021)
  • Received:2006-01-05 Revised:2006-04-28 Online:2006-11-28 Published:2006-11-28
  • Contact: WANG Huai-Sheng

Nano-porous hydroxyapatite (HAp) was prepared with sol-gel method to modify glassy carbon electrode (GCE) due to its excellent biocompatibility and particular adsorbability. Double-stranded (ds) DNA was immobilized by effective adsorption on the nano-porous hydroxyapatite modified GCE surface. The interactions of DNA immobilized on the HAp film with methylene blue (MB) were investigated by means of cyclic voltammetry (CV). The results show that the redox currents of MB at DNA modified electrode had linear relationship with scan rate from 20 to 200 mV•s-1, as expected for a surface-controlled process. The anodic and cathodic peak potentials both shifted negatively with increasing pH from 6.0 to 7.4. The results show that the interaction of DNA with MB was an electrostatic mode when ionic strength of PBS was smaller than 59 mmol•L-1, while there was not only electrostatic but also intercalative mode between them when the ionic strength of PBS was more than 59 mmol•L-1. The binding constant of MB to DNA was calculated to be 4.2×104 mol-1•L according to Langmuir adsorption formula.

Key words: DNA, nano hydroxyapatite, methylene blue, glassy carbon electrode