Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (22): 2296-2300. Previous Articles    

Reports

结合电生孔技术的抗体-纳米TiO2靶向光敏杀癌细胞

许娟1, 赵尧敏1, 陈春妹2, 孙毅1, 刘国元2, 严曼明1, 江志裕*,1   

  1. (1复旦大学化学系 分子催化与新材料重点实验室 上海 200433)
    (2复旦大学遗传学研究所 上海 200433)
  • 收稿日期:2005-12-23 修回日期:2006-05-18 出版日期:2006-11-28 发布日期:2006-11-28
  • 通讯作者: 江志裕

Oriented Photo-killing Tumor Using Antibody-nano-TiO2 Conjugates and Electroporation Methods

XU Juan1; ZHAO Yao-Min1; CHEN Chun-Mei2; SUN Yi1; LIU Guo-Yuan2; YAN Man-Ming1; JIANG Zhi-Yu*,1   

  1. (1 Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433)
    (2 Institute of Genet-ics, Fudan University, Shanghai 200433)
  • Received:2005-12-23 Revised:2006-05-18 Online:2006-11-28 Published:2006-11-28
  • Contact: JIANG Zhi-Yu

A new method combined with immunity, electroporation and nano-TiO2 photo-catalysis was successfully used to kill LoVo cancer cells. At first monoclonal antibody was adsorbed on the surface of nano-TiO2 particles, then the antibody-nano-TiO2 conjugates would adsorb on the membrane of LoVo cells spontaneously. Electroporation method was used to produce micro holes on the membrane of LoVo cells, which promoted the entering of antibody-nano-TiO2 conjugates into the cancer cells. Under the irradiation of ultra violet light, the cancer cells could be killed based on the photocatalysis effect of nano-TiO2 particles within cells. The results show that the combined technique is very efficient for killing cancer cells. In the case of using low concentration of antibody-nano-TiO2 as 3.12 μg/mL, and under the irradiation of ultra violet light at 4 mW/cm2, all of the LoVo cancer cells could be killed within 30 min. The photo-killing effi-ciency for LoVo cancer cells was much higher than that for human normal TE353.sk skin cells, indicating the high selectivity of this method. This novel combination method might be used as a new technique for the cancer treatment in the future.

Key words: human colon LoVo cell, TE353.sk cell, nano-TiO2, immunity cell electroporation, photo-ca- talysis, treatment of cancer