Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (2): 111-115. Previous Articles     Next Articles

Original Articles

Thioflavine T荧光探针法研究丝素结晶区典型肽段自聚集的β-片层结构

王建南*,1,2, 陆长德3, 白伦*,1   

  1. (1苏州大学材料工程学院 苏州 215021)
    (2苏州大学生命科学学院 苏州 215123)
    (3中国科学院上海生命科学学院生物化学与细胞生物学研究所 上海 200031)
  • 收稿日期:2006-03-11 修回日期:2006-07-09 出版日期:2007-01-28 发布日期:2007-01-28
  • 通讯作者: 王建南

Study on β-Sheet Structure Formed by Self-assembly of Fibroin Crystalline Typical Peptides Using Thioflavine T Fluorescence Probe

WANG Jian-Nan*,1,2; LU Chang-De3; BAI Lun*,1   

  1. (1 College of Material Engineering, Suzhou University, Suzhou 215021)
    (2 College of Life Science, Suzhou University, Suzhou 215123)
    (3 Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sci-ences, Shanghai 200031)
  • Received:2006-03-11 Revised:2006-07-09 Online:2007-01-28 Published:2007-01-28
  • Contact: WANG Jian-Nan

To study the self-organization/self-assembly mechanism of silkworm fibroin based material, four kinds of structurally simple fibroin crystalline peptides, GXGAGAGXGA (X: A, S, Y, V), were designed and then incubated for 1~15 d to assemble by themselves. Their abilities to form β-sheet structure were studied by ThT fluorescence spectral analysis and urea denaturation methods. Results showed that: GA could form β-sheet after incubation for 1 d; after the peptide was incubated for 12, 8, 14 and 13 d respectively (in the order of A, S, Y and V), the ratio of β-sheet structure in the aggregation tended to be stable. Among the four peptides GS had the highest β-sheet content, GA took the second place, GY and GV had the lowest content. The presence of urea in peptide solution had an obvious effect on the formation of β- sheet. Urea (1 mol/L) could stop the forming of β-sheet structure from GS, GY and GV in the process of their self-assembly. When the concentration of urea was over 2 mol/L, the formation of β-sheet structure from GA could be affected, and the content of β-sheet decreased proportionally with increase of urea concentration.

Key words: fibroin crystalline peptide, β-sheet, ThT fluorescence probe, urea