Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (7): 635-639. Previous Articles     Next Articles

Original Articles

桑蚕丝素蛋白初始结构对其矿化作用的影响

姚菊明*,1, 魏克民2, 励丽1, 孔祥东3, 祝永强2, 林凤1   

  1. (1 浙江理工大学材料与纺织学院 教育部先进纺织材料与制备技术重点实验室 杭州 310018)
    (2 浙江省中医药研究院 杭州 310007)
    (3 浙江理工大学生命科学学院 杭州 310018)
  • 收稿日期:2006-11-16 修回日期:2007-01-04 出版日期:2007-04-14 发布日期:2007-04-14
  • 通讯作者: 姚菊明

Effect of Initial Bombyx mori Silk Fibroin Structure on the Protein Biomineralization

YAO Ju-Ming*,1; WEI Ke-Min2; LI Li1; KONG Xiang-Dong3; ZHU Yong-Qiang2; LIN Feng1   

  1. (1 The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018)
    (2 Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007)
    (3 College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018)
  • Received:2006-11-16 Revised:2007-01-04 Online:2007-04-14 Published:2007-04-14
  • Contact: YAO Ju-Ming

In this study, we studied the effect of initial Bombyx mori silk fibroin structure on the protein biomineralization, where the structural transition was induced by the alkali metal ion treatment. The results from XRD, FTIR and SEM showed that the as-prepared composites formed from the silk fibroin without metal ion treatment had the sheet-like morphology, where the predominant inorganic phase was dicalcium phosphate dehydrate (DCPD). In contrast, the structural transition occurred in the silk fibroin, from random coil/helical structure to β-sheet, after the treatment of K and Na metal ions. The as-prepared composites had the fiber/rod-like morphology with the predominant inorganic phase of hydroxyapatite (HA), where the fibers/rods bundled together to form the nano-size, 3D porous network structure. It is thought that there were more hydrophilic groups with outside exposure on the extended β-sheet molecular chains, where HA crystals grew with the aggregation of silk fibroin, and further accreted onto the silk fibroin fibrils through the interaction with the hydrophilic groups. These results may provide some information on the protein biomineralization and its bio-molecular control. Moreover, the results may reflect the role of these trace elements to the bone formation, which are somewhat important for the bone repairing on clinical practice.

Key words: Bombyx mori silk fibroin, alkali metal ion, calcium phosphate, hydroxyapatite (HA), biomineralization, protein structure