Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (3): 355-361. Previous Articles     Next Articles

Original Articles

低频超声波照射下卟啉锰(HP-Mn)配合物对牛血清白蛋白(BSA)损伤的研究

王晓芳1,王君*,1,2,张朝红3,熊大珍2,陈立江1,徐锐2,张向东2   

  1. 辽宁大学化学学院 辽宁大学 辽宁大学环境科学系 辽宁大学化学系
  • 收稿日期:2007-06-08 修回日期:2007-09-13 出版日期:2008-02-14 发布日期:2008-02-14
  • 通讯作者: 王君

Investigation on damage of bovine serum albumin (BSA) by porphyrin-Mn (HP-Mn) complex under low frequency ultrasonic irradiation

WANG Xiao-Fang1; WANG Jun*,1,2 ;ZHANG Zhao-Hong3 ;XIONG Da-Zhen2; CHEN Li-Jiang1; XU Rui2 ZHANG Xiang-Dong2   

  1. (1 Department of Pharmacy, Liaoning University, Shenyang 110036)
    (2 Department of Chemistry, Liaoning University, Shenyang 110036)
    (3 Department of Environmental Science, Liaoning University, Shenyang 110036)
  • Received:2007-06-08 Revised:2007-09-13 Online:2008-02-14 Published:2008-02-14
  • Contact: WANG Jun

The interaction between hematopotphyrin-Mn (HP-Mn) complex and bovine serum albumin (BSA) as well as the damage of BSA in the presence of HP-Mn complex under ultrasonic irradiation were studied by UV-vis and fluorescence spectra. In addition, the influence of ultrasonic irradiation time, HP-Mn concentration, solution acidity (pH), ionic kind and strength were also detected. The results showed that the quenching of fluorescence of BSA caused by HP-Mn complex belonged to the static quenching. HP-Mn complex and BSA interact each other mainly through electrostatic and coordinate actions and the binding distance (r) is 3.49 nm. Otherwise, under ultrasonic irradiation the BSA molecules were obviously damaged by HP-Mn complex. The damage degree rose up with the increase of ultrasonic irradiation time, solution acidity (pH) and HP-Mn concentration, while the influence of ionic kind and strength were complicated. The research results are of great significance for driving sonodynamic treatment (SDT) to treat tumour in clinic application.

Key words: ultrasound, hematoporphyrin-Mn (HP-Mn) complex, bovine serum albumin (BSA), damage