Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (03): 205-210.     Next Articles

Full Papers

乌头类双酯型生物碱对嗜热四膜虫生长代谢的微量热学研究

唐慧英1,3,韩玉梅1,2,鄢丹*,1,张少锋1,3,罗云1,2,肖小河1,刘荣华3,彭成*,2   

  1. (1解放军中药研究所 解放军第三零二医院 北京 100039)
    (2成都中医药大学药学院 成都 610075)
    (3江西中医学院现代中药制剂教育部重点实验室 南昌 330004)
  • 收稿日期:2009-08-30 修回日期:2009-09-18 出版日期:2010-02-14 发布日期:2010-02-20
  • 通讯作者: 彭成 E-mail:yd277@126.com
  • 基金资助:

    国家“重大新药创制”科技重大专项课题(No.2009ZX09502-003);国家自然科学基金项目(No.30873385)

Microcalorimetric Investigation of Toxic Effects of Diester Diterpenoid Alkaloids from Aconiti Class on Tetrahymena thermophila BF5 Growth

Tang Huiying1,3 Han Yumei1,2 Yan Dan*,1 Zhang Shaofeng1,3 Luo Yun1,2 Xiao Xiaohe1 Liu Ronghua3 Peng Cheng*,2   

  1. (1 Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039)
    (2 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075)
    (3 Key Laboratory of Modern Preparation of TCM (Jiangxi University of Traditional Chinese Medicine), Ministry of Education, Nanchang 330004)
  • Received:2009-08-30 Revised:2009-09-18 Online:2010-02-14 Published:2010-02-20

The effect of diester diterpenoid alkaloids (DDA) from aconiti class on the growth of Tetrahymena thermophila BF5 was investigated by means of the TAM III isothermal microcalorimetric system. The extent and duration of toxic effects on the metabolism were evaluated by studying the growth rate constant (k), half inhibitory ratio (IC50), maximum heat-output power (Pmax), peak time of maximum heat-output power (tp) and total heat production (Qt). Experimental results showed that the value of tp increased with increasing the concentration of DDA. Moreover, the value of k and Pmax were reduced with increasing the concentrations of the three drugs. Furthermore, the growth rate constant (k) was linked to the concentration of DDA: raconitine=0.9910 (IC50=207.7 μg•mL-1); rmesaconitine=0.9923 (IC50=412.5 μg•mL-1); rhypaconitine=0.9977 (IC50=1497 μg•mL-1). The sequence of toxic effects of the three DDA is aconitine>mesaconitine>hypaconitine. It was suggested that the hydroxy group at C(3) and ethyl group connectted with nitrogen could possibly improve the toxic effects of DDA on Tetrahymena thermophila BF5 growth.

Key words: microcalorimetry, diester diterpenoid alkaloid, Tetrahymena thermophila BF5, growth, metabolism