Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (03): 257-262. Previous Articles     Next Articles

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  1. (河南大学化学化工学院环境与分析科学研究所 开封 475004)
  • 收稿日期:2009-04-28 修回日期:2009-09-03 出版日期:2010-02-14 发布日期:2010-02-20
  • 通讯作者: 屈建莹;;

Hydrogen Peroxide Biosensor Based on Gelatin Immobilizing Horseradish Peroxidase Qu Jianying* Chen Wenjing

Qu Jianying* Chen Wenjing   

  1. (Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004)
  • Received:2009-04-28 Revised:2009-09-03 Online:2010-02-14 Published:2010-02-20

A novel hydrogen peroxide biosensor was obtained by successfully immobilizing horseradish peroxidase (HRP) on a glassy carbon (GC) electrode, which was modified with multi-walled carbon nanotubes (MWNT) and alizarin red (AR). Then the biosensor was immerged in 3% glutaraldehyde (GA) in order to overcome the swelling and improve the stability of the gelatin membrane. The experimental condition was optimized, and the electrochemistry behavior of the HRP/AR/MWNT/GC biosensor in PBS (pH 7.0) and H2O2 was discussed. The biosensor displayed rapid response and expanded linear response range from 5.0×10-6 to 1.0×10-3 mol/L (R=0.9932) with detection limit of 1.0×10-7 mol/L in PBS (pH 7.0), as well as acceptable preparation reproducibility and excellent stability.

Key words: multi-walled carbon nanotube, alizarin red, gelatin, horseradish peroxidase, glutaraldehyde