Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (03): 269-275. Previous Articles     Next Articles

Special Topic

高效毛细管电泳用于以聚环氧乙烷为筛分介质分离DNA的机理研究

王 荣*,a 贾正平*,a 董亚蕾b 陈兴国b 谢 华a 马 骏a 张 强a 张军莉a 辛晓婷a,b,c 李文斌a 王 娟a   

  1. (a兰州军区兰州总医院临床药理基地 兰州 730050) (b兰州大学化学化工学院 兰州 730000) (c兰州大学生命科学学院 兰州 730000)
  • 收稿日期:2009-03-18 修回日期:2009-07-13 出版日期:2010-02-14 发布日期:2010-02-20
  • 通讯作者: 王荣 E-mail:wangrong@shnu.edu.cn
  • 基金资助:

    国家自然基金 (NO.20775089);甘肃省自然基金 (ZS031-A25-70-E);中国博士后基金(2005037576) 资助项目

Mechanism of the DNA Separation with High Performance Capillary Electrophoresis in Poly(ethylene oxide) Matrix

Wang, Rong*,a Jia, Zhengping*,a Dong, Yaleib Chen, Xingguob Xie, Huaa Ma, Juna Zhang, Qianga Zhang, Junlia Xin, Xiaoting a,b,c Li, Wenbina Wang, Juana   

  1. (a Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou 730050) (b Chemistry College, Lanzhou University, Lanzhou 730000) (c Life Science College, Lanzhou University, Lanzhou 730000)
  • Received:2009-03-18 Revised:2009-07-13 Online:2010-02-14 Published:2010-02-20
  • Contact: Wang Rong E-mail:wangrong@shnu.edu.cn

The 12 segments of pUC19 DNA/MspI (HpaII) marker have been separated and theoretical explanation was provided. The results gave better insight into the mechanisms of separations in polymer solutions. The dependence of different parameters on DNA size, electric field and the polymer concentration was evaluated and explained. Ogston model and a linear model were used to explain the behavior of 26~147 bp DNA fragment at lower electric field intensity. But, a new linear model was established in our experiment, which included the negative linear relation of electrophoretic mobility with DNA size. It could explain the separation mechanisms of 190~501 bp DNA fragment. The data should help to optimize and direct the small and medium DNA molecule separation in capillary electrophoresis and have a great sense in analysis of the mutational site of the tumor and the product of PCR.

Key words: high performance capillary electrophoresis, non-gel sieving, DNA, poly(ethylene oxide), separation model